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Literature summary for 3.2.1.21 extracted from

  • Kalifa, S.B.; Limam, F.; Smaali, M.I.; Maugard, T.; Marzouki, M.N.
    ?-glucosidase from Sclerotinia sclerotiorum: a new and efficient purification procedure and use as a suitable marker in immuno-enzymatic assay (2007), World J. Microbiol. Biotechnol., 23, 1363-1370.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 55°C Sclerotinia sclerotiorum

Organism

Organism UniProt Comment Textmining
Sclerotinia sclerotiorum
-
isolated from sunflower Helianthus annuus in Tunisia
-

Purification (Commentary)

Purification (Comment) Organism
native beta-glu2 60fold to homogeneity by ammonium sulfate precipitation, gel filtration, anion exchange chromatography, and chromatofocusing Sclerotinia sclerotiorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
584
-
purified beta-glu2 Sclerotinia sclerotiorum

Storage Stability

Storage Stability Organism
25°C, 1% SDS or urea, 1 week, 60% remaining activity Sclerotinia sclerotiorum
25°C, 1% Tween 20 or Triton X-100, 1 week, 90% remaining activity Sclerotinia sclerotiorum
90% of beta-Glu2 activity is retained when the enzyme is incubated for 6 days in the presence of 5 mg of BSA/ml or non-specific IgG Sclerotinia sclerotiorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Sclerotinia sclerotiorum 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Sclerotinia sclerotiorum 4-nitrophenol + beta-D-xylose
-
?

Synonyms

Synonyms Comment Organism
beta-glu2
-
Sclerotinia sclerotiorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Sclerotinia sclerotiorum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
purified enzyme, half-life is 85 min Sclerotinia sclerotiorum
65
-
purified enzyme, half-life is 25 min Sclerotinia sclerotiorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Sclerotinia sclerotiorum

pI Value

Organism Comment pI Value Maximum pI Value
Sclerotinia sclerotiorum polybuffer exchanger PBE 94 TM chromatofocusing
-
4.45