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Literature summary for 3.2.1.21 extracted from

  • Gundllapalli, S.B.; Pretorius, I.S.; Cordero Otero, R.R.
    Effect of the cellulose-binding domain on the catalytic activity of a beta-glucosidase from Saccharomycopsis fibuligera (2007), J. Ind. Microbiol. Biotechnol., 34, 413-421.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant hybrid of BGL1-CBD2 domain with cellobiohydrolase CBHII in in Saccharomyces cerevisiae strain CENPK42 under the control of phosphoglycerate-kinase-1 promoter PGK1P and terminator PGK1T and yeast mating pheromone alpha-factor secretion signal MFalpha1S, subcloning in Escherichia coli Saccharomycopsis fibuligera

Protein Variants

Protein Variants Comment Organism
additional information fusion of BGL1 to the cellulose-binding domain CBD2 of Trichoderma reesei cellobiohydrolase CBHII, recombinant enzymes are constructed with single and double copies of CBD2 fused at the N-terminus of BGL1 to mimic the two-domain organization displayed by cellulolytic enzymes in nature, the mutant shows a 2-4fold increased activity compared to the wild-type enzyme Saccharomycopsis fibuligera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wild-type and mutant enzymes Saccharomycopsis fibuligera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
recombinant wild-type enzyme Saccharomycopsis fibuligera
225000 230000 recombinant mutant enzyme Saccharomycopsis fibuligera

Organism

Organism UniProt Comment Textmining
Saccharomycopsis fibuligera
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomycopsis fibuligera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Saccharomycopsis fibuligera 4-nitrophenol + beta-D-glucose
-
?
avicel + H2O
-
Saccharomycopsis fibuligera ?
-
?
cellobiose + H2O
-
Saccharomycopsis fibuligera 2 beta-D-glucose
-
?
cellopentaose + H2O
-
Saccharomycopsis fibuligera 5 D-glucose
-
?
cellotriose + H2O
-
Saccharomycopsis fibuligera 3 D-glucose
-
?
additional information BGL1 is a non-cellulolytic yeast enzyme, substrate specifiicty with beta-1,4 linkage-specific cellulosic substrates, overview Saccharomycopsis fibuligera ?
-
?

Synonyms

Synonyms Comment Organism
BGL1
-
Saccharomycopsis fibuligera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature, substrate cellobiose Saccharomycopsis fibuligera
37
-
assay at, substrate 4-nitrophenyl-beta-D-glucopyranoside Saccharomycopsis fibuligera

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
temperature profile Saccharomycopsis fibuligera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
assay at, substrate cellobiose Saccharomycopsis fibuligera
5
-
assay at, substrate 4-nitrophenyl-beta-D-glucopyranoside Saccharomycopsis fibuligera

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH profile Saccharomycopsis fibuligera