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Literature summary for 3.2.1.21 extracted from
- Characterization of a thermostable extracellular beta-glucosidase with activities of exoglucanase and transglycosylation from Paecilomyces thermophila (2008), J. Agric. Food Chem., 56, 602-608.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight inhibition | Paecilomyces sp. 'thermophila' |  |
| Ag+ | 80% inhibition at 4 mM | Paecilomyces sp. 'thermophila' | |
| D-glucono-1,5-lactone | competitive inhibition | Paecilomyces sp. 'thermophila' | |
| D-glucose | competitive inhibition | Paecilomyces sp. 'thermophila' | |
| EDTA | slight inhibition | Paecilomyces sp. 'thermophila' | |
| Hg2+ | 95.5% inhibition at 4 mM | Paecilomyces sp. 'thermophila' | |
| additional information | no or poor inhibition by Ca2+ and DTT | Paecilomyces sp. 'thermophila' | |
| SDS | slight inhibition | Paecilomyces sp. 'thermophila' | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 0.65 | - |
cellobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 0.77 | - |
gentiobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 1.06 | - |
sophorose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 1.39 | - |
Salicin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 1.45 | - |
amygdalin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paecilomyces sp. 'thermophila' | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Paecilomyces sp. 'thermophila' | |
| Cu2+ | activates slightly | Paecilomyces sp. 'thermophila' | |
| Fe2+ | activates by 36% | Paecilomyces sp. 'thermophila' | |
| Mg2+ | activates | Paecilomyces sp. 'thermophila' | |
| Mn2+ | activates | Paecilomyces sp. 'thermophila' | |
| additional information | no metalloprotein, not affected by Al3+ at 1.0 mM | Paecilomyces sp. 'thermophila' | |
| Ni2+ | activates | Paecilomyces sp. 'thermophila' | |
| Sr2+ | activates slightly | Paecilomyces sp. 'thermophila' | |
| Zn2+ | activates | Paecilomyces sp. 'thermophila' | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paecilomyces sp. 'thermophila' | - |
- |
- |
| Paecilomyces sp. 'thermophila' J18 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the extracellular enzyme contains 82.3% carbohydrate | Paecilomyces sp. 'thermophila' |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 105fold to homogeneity by anion exchange chromatography and gel filtration | Paecilomyces sp. 'thermophila' |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 97.2 | - |
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Paecilomyces sp. 'thermophila' |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. 'thermophila' | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. 'thermophila' J18 | 4-nitrophenol + beta-D-glucose | - |
? | |
| amygdalin + 2 H2O | - |
Paecilomyces sp. 'thermophila' | mandelonitrile + 2 beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Paecilomyces sp. 'thermophila' | 2 beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Paecilomyces sp. 'thermophila' J18 | 2 beta-D-glucose | - |
? | |
| cellopentaose + H2O | - |
Paecilomyces sp. 'thermophila' | 5 D-glucose | - |
? | |
| cellotetraose + 3 H2O | - |
Paecilomyces sp. 'thermophila' | 4 beta-D-glucose | - |
? | |
| cellotriose + H2O | - |
Paecilomyces sp. 'thermophila' | 3 D-glucose | - |
? | |
| daidzin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose + daidzein | - |
? | |
| genistin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose + genistein | - |
? | |
| gentiobiose + H2O | - |
Paecilomyces sp. 'thermophila' | 2 beta-D-glucose | - |
? | |
| gentiobiose + H2O | - |
Paecilomyces sp. 'thermophila' J18 | 2 beta-D-glucose | - |
? | |
| laminarin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose | - |
? | |
| lichenan + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose | - |
? | |
| additional information | the extracellular enzyme exhibits a broad substrate specificity, shows also exoglucanase and transglycosylation activities, overview | Paecilomyces sp. 'thermophila' | ? | - |
? | |
| additional information | the extracellular enzyme exhibits a broad substrate specificity, shows also exoglucanase and transglycosylation activities, overview | Paecilomyces sp. 'thermophila' J18 | ? | - |
? | |
| salicin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose + 2-(hydroxymethyl)phenol | - |
? | |
| sophorose + H2O | - |
Paecilomyces sp. 'thermophila' | 2 beta-D-glucose | - |
? | |
| sophorose + H2O | - |
Paecilomyces sp. 'thermophila' J18 | 2 beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Paecilomyces sp. 'thermophila' |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature profile | Paecilomyces sp. 'thermophila' |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
purified enzyme, quite stable up to | Paecilomyces sp. 'thermophila' |
| 70 | - |
purified enzyme, 57.9% remaining activity | Paecilomyces sp. 'thermophila' |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.7 | - |
sophorose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 4.2 | - |
gentiobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 4.5 | - |
cellobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 7.1 | - |
Salicin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 7.9 | - |
amygdalin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 13 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Paecilomyces sp. 'thermophila' |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH profile | Paecilomyces sp. 'thermophila' |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
D-glucono-1,5-lactone | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
| 73 | - |
D-glucose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
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