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Literature summary for 3.2.1.21 extracted from

  • Nijikken, Y.; Tsukada, T.; Igarashi, K.; Samejima, M.; Wakagi, T.; Shoun, H.; Fushinobu, S.
    Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium (2007), FEBS Lett., 581, 1514-1520.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged isozyme BGL1A in Escherichia coli Phanerodontia chrysosporium

Crystallization (Commentary)

Crystallization (Comment) Organism
isozyme BGL1A in substrate-free and gluconolactone complexed forms, sitting-drop vapor diffusion method at 25 °C, using a mixture of 0.001 ml of protein solution containing 10 mg/ml protein in 20 mM Tris–HCl, pH 7.5, 150 mM NaCl, with or without 10 mM D-glucono-1,5-lactone, and 0.001 ml of a reservoir solution containing 12% isopropanol, 15% PEG 6000 and 0.1M sodium citrate, pH 5.8, the cryoprotectant solution contains 20% MPD, 15% PEG 6000 and 0.1M sodium citrate, pH 5.8, X-ray diffraction structure determination and analysis at 1.5-1.9 A resolution, modeling Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
recombinant isozyme BGL1A, gel filtration Phanerodontia chrysosporium
53000
-
1 * 53000, recombinant isozyme BGL1A, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Phanerodontia chrysosporium
-
2 beta-D-glucose
-
?
D-cellobiono-1,5-lactone + H2O Phanerodontia chrysosporium
-
D-glucose + D-glucono-1,5-lactone
-
?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q25BW5 isozyme BGL1A; isozymes BGL1A and BGL1B
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme BGL1A from Escherichia coli by anion exchange and affinity chromatography, and gel filtration Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O
-
Phanerodontia chrysosporium 2 beta-D-glucose
-
?
cellobiose + H2O substrate of isozyme BGL1B, but not of isozyme BGL1A Phanerodontia chrysosporium 2 beta-D-glucose
-
?
D-cellobiono-1,5-lactone + H2O
-
Phanerodontia chrysosporium D-glucose + D-glucono-1,5-lactone
-
?
D-cellobiono-1,5-lactone + H2O substrate of isozyme BGL1B, but not of isozyme BGL1A Phanerodontia chrysosporium D-glucose + D-glucono-1,5-lactone
-
?
additional information exo-type glycoside hydrolase that cleaves beta-glucosidic bonds from the non-reducing ends of their substrates Phanerodontia chrysosporium ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 53000, recombinant isozyme BGL1A, SDS-PAGE Phanerodontia chrysosporium
More determination and analysis of the subsite -1, i.e. glycone site, of isozyme BGL1A, overview, the loop region covering on the (beta/alpha)8 barrel is significantly deviated forming a unique subsite +1, i.e. aglycone site, of BGL1A Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
beta-glucosidase 1
-
Phanerodontia chrysosporium
BGL1
-
Phanerodontia chrysosporium
More the enzyme belongs to the glycosyl hydrolase family 1 Phanerodontia chrysosporium