Cloned (Comment) | Organism |
---|---|
expression of His-tagged isozyme BGL1A in Escherichia coli | Phanerodontia chrysosporium |
Crystallization (Comment) | Organism |
---|---|
isozyme BGL1A in substrate-free and gluconolactone complexed forms, sitting-drop vapor diffusion method at 25 °C, using a mixture of 0.001 ml of protein solution containing 10 mg/ml protein in 20 mM TrisHCl, pH 7.5, 150 mM NaCl, with or without 10 mM D-glucono-1,5-lactone, and 0.001 ml of a reservoir solution containing 12% isopropanol, 15% PEG 6000 and 0.1M sodium citrate, pH 5.8, the cryoprotectant solution contains 20% MPD, 15% PEG 6000 and 0.1M sodium citrate, pH 5.8, X-ray diffraction structure determination and analysis at 1.5-1.9 A resolution, modeling | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
recombinant isozyme BGL1A, gel filtration | Phanerodontia chrysosporium |
53000 | - |
1 * 53000, recombinant isozyme BGL1A, SDS-PAGE | Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Phanerodontia chrysosporium | - |
2 beta-D-glucose | - |
? | |
D-cellobiono-1,5-lactone + H2O | Phanerodontia chrysosporium | - |
D-glucose + D-glucono-1,5-lactone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q25BW5 | isozyme BGL1A; isozymes BGL1A and BGL1B | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme BGL1A from Escherichia coli by anion exchange and affinity chromatography, and gel filtration | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | - |
Phanerodontia chrysosporium | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | substrate of isozyme BGL1B, but not of isozyme BGL1A | Phanerodontia chrysosporium | 2 beta-D-glucose | - |
? | |
D-cellobiono-1,5-lactone + H2O | - |
Phanerodontia chrysosporium | D-glucose + D-glucono-1,5-lactone | - |
? | |
D-cellobiono-1,5-lactone + H2O | substrate of isozyme BGL1B, but not of isozyme BGL1A | Phanerodontia chrysosporium | D-glucose + D-glucono-1,5-lactone | - |
? | |
additional information | exo-type glycoside hydrolase that cleaves beta-glucosidic bonds from the non-reducing ends of their substrates | Phanerodontia chrysosporium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53000, recombinant isozyme BGL1A, SDS-PAGE | Phanerodontia chrysosporium |
More | determination and analysis of the subsite -1, i.e. glycone site, of isozyme BGL1A, overview, the loop region covering on the (beta/alpha)8 barrel is significantly deviated forming a unique subsite +1, i.e. aglycone site, of BGL1A | Phanerodontia chrysosporium |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase 1 | - |
Phanerodontia chrysosporium |
BGL1 | - |
Phanerodontia chrysosporium |
More | the enzyme belongs to the glycosyl hydrolase family 1 | Phanerodontia chrysosporium |