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Literature summary for 3.2.1.21 extracted from

  • Amouri, B.; Gargouri, A.
    Characterization of a novel beta-glucosidase from a Stachybotrys strain (2006), Biochem. Eng. J., 32, 191-197.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by SDS and 2-mercaptoethanol as long as the enzyme is not preheated Stachybotrys sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
4-nitrophenyl-beta-D-glucopyranoside pH 4.8, 50°C Stachybotrys sp.
2.22
-
cellobiose pH 4.8, 50°C Stachybotrys sp.
6.2
-
carboxymethylcellulose pH 4.8, 50°C Stachybotrys sp.
37.14
-
Salicin pH 4.8, 50°C Stachybotrys sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Stachybotrys sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
2 * 85000, SDS-PAGE Stachybotrys sp.
170000
-
about, gel filtration Stachybotrys sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Stachybotrys sp.
-
2 beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Stachybotrys sp.
-
mutant strain A19 is isolated from the cellulolytic fungus strain N1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from culture supernatant to homogeneity by anion exchange chromatography and gel filtration Stachybotrys sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information the fungus is fed-batch cultivated on cellulose Stachybotrys sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Stachybotrys sp. 4-methylumbelliferol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Stachybotrys sp. 4-nitrophenol + beta-D-glucose
-
?
carboxymethylcellulose + H2O
-
Stachybotrys sp. beta-D-glucose + 2-(hydroxymethyl)phenol
-
?
cellobiose + H2O
-
Stachybotrys sp. 2 beta-D-glucose
-
?
salicin + H2O low activity Stachybotrys sp. ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 85000, SDS-PAGE Stachybotrys sp.

Synonyms

Synonyms Comment Organism
aryl beta-glucosidase
-
Stachybotrys sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Stachybotrys sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
100 50% of maximal activity at 0°C, 30°C, and 100°C Stachybotrys sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
inactivation of the purified enzyme after 15 min Stachybotrys sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Stachybotrys sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 9 50% of maximal activity at pH 3.0 and pH 9.0 Stachybotrys sp.