Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by SDS and 2-mercaptoethanol as long as the enzyme is not preheated | Stachybotrys sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 4.8, 50°C | Stachybotrys sp. | |
2.22 | - |
cellobiose | pH 4.8, 50°C | Stachybotrys sp. | |
6.2 | - |
carboxymethylcellulose | pH 4.8, 50°C | Stachybotrys sp. | |
37.14 | - |
Salicin | pH 4.8, 50°C | Stachybotrys sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Stachybotrys sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
2 * 85000, SDS-PAGE | Stachybotrys sp. |
170000 | - |
about, gel filtration | Stachybotrys sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Stachybotrys sp. | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stachybotrys sp. | - |
mutant strain A19 is isolated from the cellulolytic fungus strain N1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from culture supernatant to homogeneity by anion exchange chromatography and gel filtration | Stachybotrys sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the fungus is fed-batch cultivated on cellulose | Stachybotrys sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Stachybotrys sp. | 4-methylumbelliferol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Stachybotrys sp. | 4-nitrophenol + beta-D-glucose | - |
? | |
carboxymethylcellulose + H2O | - |
Stachybotrys sp. | beta-D-glucose + 2-(hydroxymethyl)phenol | - |
? | |
cellobiose + H2O | - |
Stachybotrys sp. | 2 beta-D-glucose | - |
? | |
salicin + H2O | low activity | Stachybotrys sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 85000, SDS-PAGE | Stachybotrys sp. |
Synonyms | Comment | Organism |
---|---|---|
aryl beta-glucosidase | - |
Stachybotrys sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Stachybotrys sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
100 | 50% of maximal activity at 0°C, 30°C, and 100°C | Stachybotrys sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
inactivation of the purified enzyme after 15 min | Stachybotrys sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Stachybotrys sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | 50% of maximal activity at pH 3.0 and pH 9.0 | Stachybotrys sp. |