Cloned (Comment) | Organism |
---|---|
methanol-inducible expression of wild-type and mutant enzymes in Pichia pastoris strain X-33 | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
W49A | site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview | Aspergillus niger |
W49D | site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview | Aspergillus niger |
W49G | site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview | Aspergillus niger |
W49N | site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | competitive inhibition of wild-type and mutant enzymes | Aspergillus niger | |
D-gluconolactone | competitive inhibition of wild-type enzyme and mutant W49A | Aspergillus niger | |
D-glucose | competitive inhibition of wild-type and mutant enzymes | Aspergillus niger | |
gentiobiose | competitive inhibition of wild-type enzyme and mutant W49A | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of hydrolytic activity of mutant enzymes and of transglucosylation activities of recombinant wild-type and mutant enzymes, overview | Aspergillus niger | |
0.27 | - |
cellotetraose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
0.33 | - |
cellopentaose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
0.52 | - |
cellotriose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
1.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
2.2 | - |
gentiobiose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
2.7 | - |
cellobiose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Aspergillus niger | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
celloheptaose + 6 H2O = 7 beta-D-glucose | Trp49 is important for transglucosylation catalytic activity | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Aspergillus niger | 2 beta-D-glucose | - |
? | |
cellopentaose + H2O | - |
Aspergillus niger | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Aspergillus niger | 4 beta-D-glucose | - |
? | |
cellotriose + H2O | - |
Aspergillus niger | 3 D-glucose | - |
? | |
gentiobiose + H2O | - |
Aspergillus niger | 2 beta-D-glucose | - |
? | |
additional information | the enzyme shows transglucosylation activity involving Trp49, overview | Aspergillus niger | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycosyl hydrolase family 3 | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | cellotriose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
51.7 | - |
cellopentaose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
54.7 | - |
cellotetraose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
70 | - |
gentiobiose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
85.1 | - |
cellotriose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
89 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
103 | - |
cellobiose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Aspergillus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-gluconolactone | pH 4.5, 25°C, recombinant mutant W49A | Aspergillus niger | |
0.4 | - |
D-gluconolactone | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
1.1 | - |
cellobiose | pH 4.5, 25°C, recombinant mutant W49D | Aspergillus niger | |
1.2 | - |
cellobiose | pH 4.5, 25°C, recombinant mutant W49N | Aspergillus niger | |
1.3 | - |
cellobiose | pH 4.5, 25°C, recombinant mutant W49G | Aspergillus niger | |
1.4 | - |
cellobiose | pH 4.5, 25°C, recombinant mutant W49A | Aspergillus niger | |
1.6 | - |
cellobiose | pH 4.5, 25°C, recombinant mutants W49N and W49G | Aspergillus niger | |
1.8 | - |
gentiobiose | pH 4.5, 25°C, recombinant mutant W49A | Aspergillus niger | |
1.8 | - |
cellobiose | pH 4.5, 25°C, recombinant wild-type enzyme and mutant W49A | Aspergillus niger | |
1.9 | - |
cellobiose | pH 4.5, 25°C, recombinant mutant W49D | Aspergillus niger | |
3 | - |
D-glucose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger | |
4.8 | - |
gentiobiose | pH 4.5, 25°C, recombinant wild-type enzyme | Aspergillus niger |