Cloned (Comment) | Organism |
---|---|
gene bglu1, expression in in Escherichia coli with N-terminal thioredoxin and hexahistidine tags | Oryza sativa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, free and in a complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside inhibitor, growth of small crystals in 18% w/v PEG 8000 with 0.1 M sodium cacodylate, pH 6.5, and 0.2 M zinc acetate, 1-2 days, microseeding into 23% w/v PEG MME 5000, 0.2 M ammonium sulfate, and 0.1 M MES, pH 6.7, resulting in larger crystals, 1-2 months, X-ray diffraction structure determination and analysis at 2.15 A and 2.75 A resolution, respectively, molecular replacement, screening and method optimization, overview | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside | - |
Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE | Oryza sativa |
66000 | - |
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q75I93 | subsp. japonica, gene bglu1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by immobilized metal-affinity chromatography and removal of the tags, followed by cation exchange chromatography and gel filtration to about 95% purity | Oryza sativa |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
BGlu1 | - |
Oryza sativa |
More | the enzyme belongs to the glycosyl hydrolase family 1 | Oryza sativa |