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Literature summary for 3.2.1.21 extracted from

  • Chuenchor, W.; Pengthaisong, S.; Yuvaniyama, J.; Opassiri, R.; Svasti, J.; Ketudat Cairns, J.R.
    Purification, crystallization and preliminary X-ray analysis of rice BGlu1 beta-glucosidase with and without 2-deoxy-2-fluoro-beta-D-glucoside (2006), Acta Crystallogr. Sect. F, 62, 798-801.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene bglu1, expression in in Escherichia coli with N-terminal thioredoxin and hexahistidine tags Oryza sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, free and in a complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside inhibitor, growth of small crystals in 18% w/v PEG 8000 with 0.1 M sodium cacodylate, pH 6.5, and 0.2 M zinc acetate, 1-2 days, microseeding into 23% w/v PEG MME 5000, 0.2 M ammonium sulfate, and 0.1 M MES, pH 6.7, resulting in larger crystals, 1-2 months, X-ray diffraction structure determination and analysis at 2.15 A and 2.75 A resolution, respectively, molecular replacement, screening and method optimization, overview Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside
-
Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE Oryza sativa
66000
-
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q75I93 subsp. japonica, gene bglu1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by immobilized metal-affinity chromatography and removal of the tags, followed by cation exchange chromatography and gel filtration to about 95% purity Oryza sativa

Subunits

Subunits Comment Organism
? x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
BGlu1
-
Oryza sativa
More the enzyme belongs to the glycosyl hydrolase family 1 Oryza sativa