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Literature summary for 3.2.1.21 extracted from

  • Murray, P.; Aro, N.; Collins, C.; Grassick, A.; Penttilae, M.; Saloheimo, M.; Tuohy, M.
    Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersonii (2004), Protein Expr. Purif., 38, 248-257.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cel3a, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli, expression in Trichoderma reesei Rasamsonia emersonii

Inhibitors

Inhibitors Comment Organism Structure
D-glucose competitive inhibition Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 72°C, recombinant enzyme Rasamsonia emersonii
0.18
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 72°C, recombinant enzyme Aspergillus niger
1.1
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 72°C, recombinant enzyme Trichoderma reesei

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Rasamsonia emersonii 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
2 * 130000, recombinant enzyme, SDS-PAGE Rasamsonia emersonii
260000
-
native PAGE and gel filtration, recombinant enzyme Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Aspergillus niger Q9P8F4
-
-
Rasamsonia emersonii Q8TGI8 gene cel3a
-
Rasamsonia emersonii CBS 814.70 Q8TGI8 gene cel3a
-
Trichoderma reesei Q7Z9M5 gene cel3b
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 17 potental N-glycosylation sites Rasamsonia emersonii

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 24fold from Trichoderma reesei strain Rut-C30 by ultrafiltration, two steps of gel filtration, and ion exchange chromatography Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
482.8
-
purified recombinant enzyme Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Rasamsonia emersonii 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Rasamsonia emersonii CBS 814.70 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus niger 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Trichoderma reesei 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus niger 4-nitrophenol + beta-D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Trichoderma reesei 4-nitrophenol + beta-D-glucopyranose
-
?
cellobiose + H2O
-
Rasamsonia emersonii 2 D-glucose
-
?
cellobiose + H2O
-
Rasamsonia emersonii CBS 814.70 2 D-glucose
-
?
cellooligosaccharides + H2O
-
Rasamsonia emersonii beta-D-glucose
-
?
cellooligosaccharides + H2O
-
Rasamsonia emersonii CBS 814.70 beta-D-glucose
-
?
additional information substrate specificity, barley glucan and 4-nitrophenyl beta-cellobioside are poor substrates, no activity with lactose, carboxy methyl cellulose, xyloglucan, and lichenan, the enzyme is a specific beta-glucosidase and does not show beta-galactosidase activity, but it shows additional glucosyl transferase activity producing cellobiose from D-glucose and cellotetrose from cellobiose Rasamsonia emersonii ?
-
?
additional information substrate specificity, barley glucan and 4-nitrophenyl beta-cellobioside are poor substrates, no activity with lactose, carboxy methyl cellulose, xyloglucan, and lichenan, the enzyme is a specific beta-glucosidase and does not show beta-galactosidase activity, but it shows additional glucosyl transferase activity producing cellobiose from D-glucose and cellotetrose from cellobiose Rasamsonia emersonii CBS 814.70 ?
-
?
salicin + H2O
-
Rasamsonia emersonii saligenin + D-glucose
-
?
salicin + H2O
-
Rasamsonia emersonii CBS 814.70 saligenin + D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 130000, recombinant enzyme, SDS-PAGE Rasamsonia emersonii
More secondary structure prediction and homology molecular modeling Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
Cel3A
-
Rasamsonia emersonii
Cel3b
-
Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Aspergillus niger
70
-
-
Trichoderma reesei
71.5
-
-
Rasamsonia emersonii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80
-
Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
recombinant enzyme, half-life is 62 min Rasamsonia emersonii
75
-
recombinant enzyme, half-life is 18 min Rasamsonia emersonii
80
-
the recombinant enzyme retains 30% of maximal activity after 25 min Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2226
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 72°C, recombinant enzyme Rasamsonia emersonii
4000
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 72°C, recombinant enzyme Aspergillus niger
8664
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 72°C, recombinant enzyme Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
assay at Rasamsonia emersonii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.254
-
D-glucose pH 5.0, 72°C Rasamsonia emersonii

pI Value

Organism Comment pI Value Maximum pI Value
Rasamsonia emersonii isoelectric focusing, recombinant enzyme
-
3.6