Cloned (Comment) | Organism |
---|---|
gene cel3a, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli, expression in Trichoderma reesei | Rasamsonia emersonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | competitive inhibition | Rasamsonia emersonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Rasamsonia emersonii | |
0.18 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Aspergillus niger | |
1.1 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Trichoderma reesei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Rasamsonia emersonii | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
2 * 130000, recombinant enzyme, SDS-PAGE | Rasamsonia emersonii |
260000 | - |
native PAGE and gel filtration, recombinant enzyme | Rasamsonia emersonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | Q9P8F4 | - |
- |
Rasamsonia emersonii | Q8TGI8 | gene cel3a | - |
Rasamsonia emersonii CBS 814.70 | Q8TGI8 | gene cel3a | - |
Trichoderma reesei | Q7Z9M5 | gene cel3b | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 17 potental N-glycosylation sites | Rasamsonia emersonii |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 24fold from Trichoderma reesei strain Rut-C30 by ultrafiltration, two steps of gel filtration, and ion exchange chromatography | Rasamsonia emersonii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
482.8 | - |
purified recombinant enzyme | Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rasamsonia emersonii | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rasamsonia emersonii CBS 814.70 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
cellobiose + H2O | - |
Rasamsonia emersonii | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Rasamsonia emersonii CBS 814.70 | 2 D-glucose | - |
? | |
cellooligosaccharides + H2O | - |
Rasamsonia emersonii | beta-D-glucose | - |
? | |
cellooligosaccharides + H2O | - |
Rasamsonia emersonii CBS 814.70 | beta-D-glucose | - |
? | |
additional information | substrate specificity, barley glucan and 4-nitrophenyl beta-cellobioside are poor substrates, no activity with lactose, carboxy methyl cellulose, xyloglucan, and lichenan, the enzyme is a specific beta-glucosidase and does not show beta-galactosidase activity, but it shows additional glucosyl transferase activity producing cellobiose from D-glucose and cellotetrose from cellobiose | Rasamsonia emersonii | ? | - |
? | |
additional information | substrate specificity, barley glucan and 4-nitrophenyl beta-cellobioside are poor substrates, no activity with lactose, carboxy methyl cellulose, xyloglucan, and lichenan, the enzyme is a specific beta-glucosidase and does not show beta-galactosidase activity, but it shows additional glucosyl transferase activity producing cellobiose from D-glucose and cellotetrose from cellobiose | Rasamsonia emersonii CBS 814.70 | ? | - |
? | |
salicin + H2O | - |
Rasamsonia emersonii | saligenin + D-glucose | - |
? | |
salicin + H2O | - |
Rasamsonia emersonii CBS 814.70 | saligenin + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 130000, recombinant enzyme, SDS-PAGE | Rasamsonia emersonii |
More | secondary structure prediction and homology molecular modeling | Rasamsonia emersonii |
Synonyms | Comment | Organism |
---|---|---|
Cel3A | - |
Rasamsonia emersonii |
Cel3b | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Aspergillus niger |
70 | - |
- |
Trichoderma reesei |
71.5 | - |
- |
Rasamsonia emersonii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | - |
Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
recombinant enzyme, half-life is 62 min | Rasamsonia emersonii |
75 | - |
recombinant enzyme, half-life is 18 min | Rasamsonia emersonii |
80 | - |
the recombinant enzyme retains 30% of maximal activity after 25 min | Rasamsonia emersonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2226 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Rasamsonia emersonii | |
4000 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Aspergillus niger | |
8664 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Rasamsonia emersonii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.254 | - |
D-glucose | pH 5.0, 72°C | Rasamsonia emersonii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rasamsonia emersonii | isoelectric focusing, recombinant enzyme | - |
3.6 |