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Literature summary for 3.2.1.21 extracted from
- Kinetics of improved production and thermostability of an intracellular beta-glucosidase from a mutant-derivative of Cellulomonas biazotea (2004), Biotechnol. Lett., 26, 281-285.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the deoxyglucose- and streptomycin-resistant mutant strain shows 1.2-1.5fold higher thermostability and resistance against 4 M urea, and has a lower activation energy compared to the of parent wild-type strain NIAB 442 | Cellulomonas biazotea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | half-life of the wild-type enzyme at 4 M is 72 min, and of the mutant enzyme 126 min | Cellulomonas biazotea |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Cellulomonas biazotea | 5622 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas biazotea | - |
parent wild-type strain NIAB 442 and a deoxyglucose- and streptomycin-resistant mutant strain thereof | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzymes from wild-type strain NIAB 442, 17fold, and the deoxyglucose- and streptomycin-resistant mutant strain, 23fold, to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Cellulomonas biazotea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cells grown on straw of Leptochloa fusca, a salt-resistant grass, resulting in high enzyme activity, or on salt culture medium with different carbon sources added, overview | Cellulomonas biazotea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
mutant strain | Cellulomonas biazotea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas biazotea | 4-nitrophenol + beta-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BglB | - |
Cellulomonas biazotea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermodynamics | Cellulomonas biazotea |
| 40 | - |
assay at | Cellulomonas biazotea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | comparison of thermostability of the parent wild-type and the mutant strain at 25-75°C over 15 min | Cellulomonas biazotea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Cellulomonas biazotea |
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Release 2023.1 (January 2023)
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