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Literature summary for 3.2.1.21 extracted from
- Substrate specificity of Aspergillus oryzae family 3 beta-glucosidase (2006), Biochim. Biophys. Acta, 1764, 972-978.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucoheptose | competitive inhibition | Aspergillus oryzae |  |
| D-glucose | competitive inhibition | Aspergillus oryzae | |
| D-glucuronamide | competitive inhibition | Aspergillus oryzae | |
| D-mannose | competitive inhibition | Aspergillus oryzae | |
| D-sorbose | competitive inhibition | Aspergillus oryzae | |
| deoxynojirimycin | competitive inhibition | Aspergillus oryzae | |
| gluconolactone | competitive inhibition | Aspergillus oryzae | |
| L-idose | competitive inhibition | Aspergillus oryzae | |
| additional information | IC50 values, overview | Aspergillus oryzae | |
| myo-inositol | competitive inhibition | Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Aspergillus oryzae | |
| 0.29 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 40°C | Aspergillus oryzae | |
| 0.36 | - |
laminaribiose | pH 5.0, 40°C | Aspergillus oryzae | |
| 0.7 | - |
beta-gentiobiose | pH 5.0, 40°C | Aspergillus oryzae | |
| 1.96 | - |
cellobiose | pH 5.0, 40°C | Aspergillus oryzae | |
| 2.48 | - |
sophorose | pH 5.0, 40°C | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
x * 120000, SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
family 3 enzyme | - |
| Aspergillus oryzae GH3 | - |
family 3 enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by chromatographic steps to over 95% purity | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 176 | - |
purified enzyme, substrate 4-nitrophenyl beta-D-glucopyranoside, 60°C | Aspergillus oryzae |
| 350 | - |
purified enzyme, substrate cellobiose, 40°C | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae GH3 | 4-nitrophenol + beta-D-glucose | - |
? | |
| beta-gentiobiose + H2O | - |
Aspergillus oryzae | 2 D-glucose | - |
? | |
| beta-gentiobiose + H2O | - |
Aspergillus oryzae GH3 | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Aspergillus oryzae | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Aspergillus oryzae GH3 | 2 D-glucose | - |
? | |
| laminaribiose + H2O | - |
Aspergillus oryzae | D-glucose | - |
? | |
| laminaribiose + H2O | - |
Aspergillus oryzae GH3 | D-glucose | - |
? | |
| additional information | substrate specificity, overview | Aspergillus oryzae | ? | - |
? | |
| additional information | substrate specificity, overview | Aspergillus oryzae GH3 | ? | - |
? | |
| sophorose + H2O | - |
Aspergillus oryzae | 2 D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 120000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BG | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 65 | - |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable for 1 h | Aspergillus oryzae |
| 70 | - |
inactivation | Aspergillus oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 370 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 40°C | Aspergillus oryzae | |
| 820 | - |
sophorose | pH 5.0, 40°C | Aspergillus oryzae | |
| 930 | - |
laminaribiose | pH 5.0, 40°C | Aspergillus oryzae | |
| 950 | - |
beta-gentiobiose | pH 5.0, 40°C | Aspergillus oryzae | |
| 1000 | - |
cellobiose | pH 5.0, 40°C | Aspergillus oryzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | 67% of maximal activity at pH 4.0, 85% at pH 6.0 | Aspergillus oryzae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
deoxynojirimycin | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 0.07 | - |
gluconolactone | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 2.9 | - |
D-glucose | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 54 | - |
D-mannose | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 130 | - |
D-glucuronamide | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 160 | - |
D-sorbose | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 330 | - |
D-glucoheptose | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 360 | - |
L-idose | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
| 440 | - |
myo-inositol | pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside | Aspergillus oryzae | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus oryzae | sequence calculation | - |
4.9 |
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