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Literature summary for 3.2.1.21 extracted from

  • Seidle, H.F.; Marten, I.; Shoseyov, O.; Huber, R.E.
    Physical and kinetic properties of the family 3 beta-glucosidase from Aspergillus niger which is important for cellulose breakdown (2004), Protein J., 23, 11-23.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
cellobiose
-
 Aspergillus niger
cellotriose
-
 Aspergillus niger
D-allose
-
 Aspergillus niger
D-galactose
-
 Aspergillus niger
D-gluconolactone
-
 Aspergillus niger
D-glucose
-
 Aspergillus niger
D-mannose
-
 Aspergillus niger
D-xylose
-
 Aspergillus niger
gentiobiose
-
 Aspergillus niger
maltose
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 cellotetraose 25°C, pH 4.5 Aspergillus niger
0.4
-
 cellopentaose 25°C, pH 4.5 Aspergillus niger
0.5
-
 cellotriose 25°C, pH 4.5 Aspergillus niger
1
-
 p-nitrophenyl-beta-D-glucopyranoside 25°C, pH 4.5 Aspergillus niger
1.3
-
 gentiobiose 25°C, pH 4.5 Aspergillus niger
1.5
-
 laminaribose 25°C, pH 4.5 Aspergillus niger
1.5
-
 4-O-gentiobiosyl-glucose 25°C, pH 4.5 Aspergillus niger
2.7
-
 cellobiose 25°C, pH 4.5 Aspergillus niger
3.2
-
 o-nitrophenyl-beta-D-glucopyranoside 25°C, pH 4.5 Aspergillus niger
3.9
-
 sophorose 25°C, pH 4.5 Aspergillus niger
4.6
-
 isotrehalose 25°C, pH 4.5 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91400
-
 2 * 91400, deduced from nucleotide sequence Aspergillus niger
120000
-
 2 * 120000, SDS-PAGE Aspergillus niger
210000
-
 native-PAGE Aspergillus niger
223000
-
 gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-gentiobiosyl-glucose + H2O
-
 Aspergillus niger ?
-
 ?
cellobiose + H2O Bgs also has transglucosidic activity Aspergillus niger 2 beta-D-glucose
-
 ?
cellopentaose + H2O
-
 Aspergillus niger cellotetraose + beta-D-glucose
-
 ?
cellotetraose + H2O
-
 Aspergillus niger cellotriose + D-glucose
-
 ?
cellotriose + H2O
-
 Aspergillus niger cellobiose + beta-D-glucose
-
 ?
gentiobiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
isotrehalose + H2O
-
 Aspergillus niger beta-D-glucose
-
 ?
laminaribiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
o-nitrophenyl-beta-D-glucopyranose + H2O
-
 Aspergillus niger o-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-glucopyranose + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-glucose
-
 ?
sophorose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 120000, SDS-PAGE Aspergillus niger
dimer 2 * 91400, deduced from nucleotide sequence Aspergillus niger

Synonyms

Synonyms Comment Organism
BGs
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3 6 approx. 70% of maximal activity at pH 3.0, approx. 5% of maximal activity at pH 6.0 Aspergillus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 cellotriose 25°C, pH 4.5 Aspergillus niger
0.4
-
 D-gluconolactone 25°C, pH 4.5 Aspergillus niger
3
-
 D-glucose 25°C, pH 4.5 Aspergillus niger
3.3
-
 cellobiose 25°C, pH 4.5 Aspergillus niger
4.7
-
 gentiobiose 25°C, pH 4.5 Aspergillus niger
260
-
 D-mannose 25°C, pH 4.5 Aspergillus niger
260
-
 D-allose 25°C, pH 4.5 Aspergillus niger
320
-
 maltose 25°C, pH 4.5 Aspergillus niger
340
-
 D-xylose 25°C, pH 4.5 Aspergillus niger
670
-
 D-galactose 25°C, pH 4.5 Aspergillus niger