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Literature summary for 3.2.1.21 extracted from

  • Brunner, F.; Wirtz, W.; Rose, J.K.C.; Darvill, A.G.; Govers, F.; Scheel, D.; Nurnberger, T.
    A beta-glucosidase/xylosidase from the phytopathogenic oomycete, Phytophthora infestans (2002), Phytochemistry, 59, 689-696.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Phytophthora infestans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 4-methylumbelliferyl-beta-D-glucopyranoside 37°C, pH 5.5 Phytophthora infestans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60680
-
 2 * 60680, deduced from nucleotide sequence Phytophthora infestans
85000
-
 2 * 85000, SDS-PAGE, difference to deduced molecular weight is due to glycosylation Phytophthora infestans
154000
-
 gel filtration Phytophthora infestans

Organism

Organism UniProt Comment Textmining
Phytophthora infestans Q9AT27 phytopathogenic oomycete
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Phytophthora infestans

Purification (Commentary)

Purification (Comment) Organism
-
 Phytophthora infestans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
-
 Phytophthora infestans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O 0.09% of activity with 4-methylumbelliferyl-beta-D-glucopyranoside Phytophthora infestans 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
4-methylumbelliferyl-alpha-D-glucopyranoside + H2O 0.1% of activity with 4-methylumbelliferyl-beta-D-glucopyranoside Phytophthora infestans 4-methylumbelliferone + alpha-D-glucose
-
 ?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O 0.5% of activity with 4-methylumbelliferyl-beta-D-glucopyranoside Phytophthora infestans 4-methylumbelliferone + beta-D-galactose
-
 ?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Phytophthora infestans 4-methylumbelliferone + beta-D-glucose
-
 ?
4-methylumbelliferyl-beta-D-lactopyranoside + H2O 0.06% of activity with 4-methylumbelliferyl-beta-D-glucopyranoside Phytophthora infestans 4-methylumbelliferone + beta-D-galactose + D-glucose
-
 ?
4-methylumbelliferyl-beta-D-mannopyranoside + H2O 0.075% of activity with 4-methylumbelliferyl-beta-D-glucopyranoside Phytophthora infestans 4-methylumbelliferone + beta-D-mannose
-
 ?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O 7% of activity with 4-methylumbelliferyl-beta-D-glucopyranoside Phytophthora infestans 4-methylumbelliferone + beta-D-xylose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60680, deduced from nucleotide sequence Phytophthora infestans
dimer 2 * 85000, SDS-PAGE, difference to deduced molecular weight is due to glycosylation Phytophthora infestans

Synonyms

Synonyms Comment Organism
BGX1
-
 Phytophthora infestans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Phytophthora infestans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6
-
 Phytophthora infestans

pI Value

Organism Comment pI Value Maximum pI Value
Phytophthora infestans isoelectric focusing
-
 3.5
Phytophthora infestans deduced from nucleotide sequence
-
 4.2