Literature summary for 3.2.1.21 extracted from

Berrin, J.G.; McLauchlan, W.R.; Needs, P.; Williamson, G.; Puigserver, A.; Kroon, P.A.; Juge, N.
Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the metabolism of dietary glucosides (2002), Eur. J. Biochem., 269, 249-258.

Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside	0.001 mM, 64% inhibition after 30 min incubation	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	luteolin-4'-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.022	-	apigenin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.032	-	quercetin-4'-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.035	-	genistein-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.042	-	quercetin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.05	-	luteolin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.12	-	daidzein-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.25	-	eriodictyol-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.274	-	quercetin-3,4'-di-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.37	-	p-nitrophenyl-beta-D-fucopyranoside	37°C, pH 6.5	Homo sapiens
0.43	-	naringenin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.57	-	p-nitrophenyl-alpha-L-arabinopyranoside	37°C, pH 6.5	Homo sapiens
1.58	-	p-nitrophenyl-beta-D-xylopyranoside	37°C, pH 6.5	Homo sapiens
1.76	-	p-nitrophenyl-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
3.14	-	p-nitrophenyl-beta-D-galactopyranoside	37°C, pH 6.5	Homo sapiens
3.2	-	daidzein 7-O-(6''-O-beta-D-malonyl-beta-D-glucoside)	37°C, pH 6.5	Homo sapiens
52.6	-	p-nitrophenyl-beta-L-arabinopyranoside	37°C, pH 6.5	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51900	-	1 * 51900, SDS-PAGE	Homo sapiens
53000	-	1 * 53000, SDS-PAGE, recombinant CBG	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native CBG, cation-exchange chromatography, Octyl-Sepharose, chromatofocusing, gel filtration, recombinant CBG, Octyl-Sepharose	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10	-	recombinant CBG, hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside	Homo sapiens
12.8	-	native CBG, hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
apigenin-beta-D-glucopyranoside + H2O	-	Homo sapiens	apigenin + beta-D-glucose	-	?
daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O	-	Homo sapiens	daidzein + 6-O-malonyl-beta-D-glucopyranose	-	?
daidzein-7-beta-D-glucopyranoside + H2O	-	Homo sapiens	daidzein + beta-d-glucose	-	?
eriodictyol-7-beta-D-glucopyranoside + H2O	-	Homo sapiens	eriodictyol + D-glucose	-	?
genistein-7-beta-D-glucopyranoside + H2O	-	Homo sapiens	genistein + beta-D-glucose	-	?
luteolin-3',7-dibeta-D-glucopyranoside + H2O	-	Homo sapiens	luteolin + beta-D-glucose	-	?
luteolin-4'-beta-D-glucopyranoside + H2O	-	Homo sapiens	luteolin + beta-D-glucose	-	?
luteolin-7-beta-D-glucopyranoside + H2O	-	Homo sapiens	luteolin + beta-D-glucose	-	?
naringenin-7-beta-D-glucopyranoside + H2O	-	Homo sapiens	naringenin + beta-D-glucose	-	?
p-nitrophenyl-alpha-L-arabinopyranoside + H2O	-	Homo sapiens	p-nitrophenol + alpha-L-arabinose	-	?
p-nitrophenyl-beta-D-fucopyranoside + H2O	-	Homo sapiens	p-nitrophenol + beta-D-fucose	-	?
p-nitrophenyl-beta-D-galactopyranoside + H2O	-	Homo sapiens	p-nitrophenol + beta-D-galactose	-	?
p-nitrophenyl-beta-D-glucopyranoside + H2O	-	Homo sapiens	p-nitrophenol + beta-D-glucose	-	?
p-nitrophenyl-beta-D-xylopyranoside + H2O	-	Homo sapiens	p-nitrophenol + beta-D-xylose	-	?
p-nitrophenyl-beta-L-arabinopyranoside + H2O	-	Homo sapiens	p-nitrophenol + beta-L-arabinose	-	?
quercetin 3,4'-O-di-beta-D-glucopyranoside + H2O	-	Homo sapiens	quercetin + 2 beta-D-glucose	-	?
quercetin 4'-O-beta-D-glucopyranoside + H2O	-	Homo sapiens	quercetin + beta-D-glucose	-	?
quercetin 7-O-beta-D-glucopyranoside + H2O	-	Homo sapiens	quercetin + beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 51900, SDS-PAGE	Homo sapiens
monomer	1 * 53000, SDS-PAGE, recombinant CBG	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CBG	-	Homo sapiens
reCBG	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	less than 20% loss of activity after 24 h	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	daidzein 7-O-(6''-O-beta-D-malonyl-beta-D-glucoside)	37°C, pH 6.5	Homo sapiens
0.3	-	quercetin-3,4'-di-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.66	-	p-nitrophenyl-beta-L-arabinopyranoside	37°C, pH 6.5	Homo sapiens
0.69	-	quercetin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
0.75	-	p-nitrophenyl-beta-D-xylopyranoside	37°C, pH 6.5	Homo sapiens
1.08	-	quercetin-4'-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
1.17	-	luteolin-4'-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
1.26	-	eriodictyol-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
1.53	-	genistein-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
1.53	-	apigenin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
2.6	-	naringenin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
3.05	-	luteolin-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
3.55	-	daidzein-7-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
5.97	-	p-nitrophenyl-alpha-L-arabinopyranoside	37°C, pH 6.5	Homo sapiens
10.7	-	p-nitrophenyl-beta-D-fucopyranoside	37°C, pH 6.5	Homo sapiens
12.1	-	p-nitrophenyl-beta-D-glucopyranoside	37°C, pH 6.5	Homo sapiens
17.6	-	p-nitrophenyl-beta-D-galactopyranoside	37°C, pH 6.5	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	-	less than 4% of maximal activity	Homo sapiens
5	7.5	more than 70% of maximal activity	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	isoelectric focusing	-	4.9

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Release 2023.1 (January 2023)