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Literature summary for 3.2.1.21 extracted from

  • Park, S.Y.; Bae, E.A.; Sung, J.H.; Lee, S.K.; Kim, D.H.
    Purification and characterization of ginsenoside Rb1-metabolizing beta-glucosidase from Fusobacterium K-60, a human intestinal anaerobic bacterium (2001), Biosci. Biotechnol. Biochem., 65, 1163-1169.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanediol more than2 mM, 50% inhibition Fusobacterium sp.
Ba2+ 63% inhibition Fusobacterium sp.
carbodiimide more than 2 mM, 50% inhibition Fusobacterium sp.
D-glucose 6.8 mM, 50% inhibition Fusobacterium sp.
D-mannose 5.1 mM, 50% inhibition Fusobacterium sp.
dithiothreitol more than 2 mM, 50% inhibition Fusobacterium sp.
Fe2+ 57% inhibition Fusobacterium sp.
iodoacetic acid more than 2 mM, 50% inhibition Fusobacterium sp.
Li+ 40% inhibition Fusobacterium sp.
N-ethylmaleinimide more than 2 mM, 50% inhibition Fusobacterium sp.
p-chloromercuryphenylsulfonic acid 0.0015 mM, 50% inhibition Fusobacterium sp.
paraoxon 1.7 mM, 50% inhibition Fusobacterium sp.
saccharic acid 1,4-lacotone 3.7 mM, 50% inhibition Fusobacterium sp.
tosyl-L-lysine chlormethyl ketone more than 2 mM, 50% inhibition Fusobacterium sp.
Tris 6.7 mM, 50% inhibition Fusobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 p-nitrophenyl-beta-D-glucopyranoside 37°C, pH 6.0 Fusobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 4 * 80000, SDS-PAGE Fusobacterium sp.
320000
-
 gel filtration Fusobacterium sp.

Organism

Organism UniProt Comment Textmining
Fusobacterium sp.
-
 K-60, human intestinal bacterium
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Butyl-Toyopearl, hydroxyapatite, Q-Sepharose, Sephacryl S-300 Fusobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.52
-
-
 Fusobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amygdalin + 2 H2O 22% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. mandelonitrile + 2 beta-D-glucose
-
 ?
cellobiose + H2O 3.6% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. 2 beta-D-glucose
-
 ?
esculin + H2O 100% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. esculetin + beta-D-glucose
-
 ?
gentiobiose + H2O 2.5% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. 2 beta-D-glucose
-
 ?
ginsenoside Rb1 + H2O 63% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. ? + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Fusobacterium sp. p-nitrophenol + beta-D-glucose
-
 ?
sophorose + H2O 144% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. 2 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 80000, SDS-PAGE Fusobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 at 37°C Fusobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 approx. 5% of maximal activity at pH 4.0 and pH 8.5, respectively Fusobacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Fusobacterium sp. chromatofocusing
-
 5.25