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Literature summary for 3.2.1.21 extracted from

  • Parry, N.J.; Beever, D.E.; Owen, E.; Vandenberghe, I.; Van Beeumen, J.; Bhat, M.K.
    Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus (2001), Biochem. J., 353, 117-127.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ethanol 30%, 40% activation Thermoascus aurantiacus
methanol 30%, 30% activation Thermoascus aurantiacus
propan-2-ol concentrations below 50%, activation Thermoascus aurantiacus

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose
-
Thermoascus aurantiacus
D-delta-gluconolactone
-
Thermoascus aurantiacus
ethanol inhibition above 50% concentration Thermoascus aurantiacus
methanol inhibition above 50% concentration Thermoascus aurantiacus
propan-2-ol inhibition above 50% concentration, 50% inhibition at 90%-100% propan-2-ol Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
3 * 120000, SDS-PAGE Thermoascus aurantiacus
350000
-
gel filtration Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
IMI216529
-
Thermoascus aurantiacus IMI216529
-
IMI216529
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, Ultrogel AcA 44, Mono P Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
190000
-
at pH 4.5 and 60°C Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-cellobiose + H2O
-
Thermoascus aurantiacus 4-methylumbelliferyl-beta-D-glucopyranoside + beta-D-glucose
-
?
4-methylumbelliferyl-beta-D-cellobiose + H2O
-
Thermoascus aurantiacus IMI216529 4-methylumbelliferyl-beta-D-glucopyranoside + beta-D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Thermoascus aurantiacus 4-methylumbelliferol + beta-D-glucose
-
?
arbutin + H2O
-
Thermoascus aurantiacus beta-D-glucose + ?
-
?
beta-beta-trehalose + H2O
-
Thermoascus aurantiacus beta-D-glucose
-
?
cellobiose + H2O
-
Thermoascus aurantiacus 2 beta-D-glucose
-
?
cellobiose + H2O
-
Thermoascus aurantiacus IMI216529 2 beta-D-glucose
-
?
cellobiose + methanol 25% methanol, transglycosylation activity Thermoascus aurantiacus methyl-beta-D-glucopyranoside + beta-D-glucose
-
?
CM-cellulose + H2O
-
Thermoascus aurantiacus CM-cellulose + beta-D-glucose
-
?
esculin + H2O
-
Thermoascus aurantiacus beta-D-glucose + ?
-
?
gentiobiose + H2O
-
Thermoascus aurantiacus 2 beta-D-glucose
-
?
gentiobiose + H2O
-
Thermoascus aurantiacus IMI216529 2 beta-D-glucose
-
?
laminarin + H2O 100times lower activity than with cellobiose Thermoascus aurantiacus laminarioligosaccharides + D-glucose
-
?
methyl-beta-D-glucopyranoside + H2O
-
Thermoascus aurantiacus methanol + beta-D-glucopyranose
-
?
n-octyl-beta-D-glucopyranoside + H2O
-
Thermoascus aurantiacus n-octanol + beta-D-glucose
-
?
o-nitrophenyl-beta-D-glucopyranoside + H2O
-
Thermoascus aurantiacus o-nitrophenol + beta-D-glucose
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
Thermoascus aurantiacus p-nitrophenol + beta-D-glucose
-
?
salicin + H2O
-
Thermoascus aurantiacus beta-D-glucose + ?
-
?
salicin + H2O
-
Thermoascus aurantiacus IMI216529 beta-D-glucose + ?
-
?
sophorose + H2O
-
Thermoascus aurantiacus 2 beta-D-glucose
-
?
sophorose + H2O
-
Thermoascus aurantiacus IMI216529 2 beta-D-glucose
-
?

Subunits

Subunits Comment Organism
trimer 3 * 120000, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermoascus aurantiacus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 70% of maximal activity at 90°C, low activity below 60°C Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
no loss of activity after 48 h at pH 6.8 Thermoascus aurantiacus
70
-
no loss of activity after 30 min at pH 5.2 Thermoascus aurantiacus
80
-
90% loss of activity after 30 min at pH 5.2 Thermoascus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
136.4
-
sophorose 60°C, pH 4.5 Thermoascus aurantiacus
140.7
-
gentiobiose 60°C, pH 4.5 Thermoascus aurantiacus
234.8
-
n-octyl-beta-D-glucopyranoside 60°C, pH 4.5 Thermoascus aurantiacus
235.2
-
o-nitrophenyl-beta-D-glucopyranoside 60°C, pH 4.5 Thermoascus aurantiacus
242.2
-
p-nitrophenyl-beta-D-glucopyranoside 60°C, pH 4.5 Thermoascus aurantiacus
284.2
-
cellobiose 60°C, pH 4.5 Thermoascus aurantiacus
284.2
-
beta,beta-trehalose 60°C, pH 4.5 Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Thermoascus aurantiacus

pH Range

pH Minimum pH Maximum Comment Organism
2.8 6.8 approx. 30% of maximal activity at pH 3.5 and pH 6.8, respectively Thermoascus aurantiacus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0083
-
D-delta-gluconolactone 60°C, pH 4.5 Thermoascus aurantiacus
0.29
-
beta-D-glucose 60°C, pH 4.5 Thermoascus aurantiacus