Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 30%, 40% activation | Thermoascus aurantiacus | |
methanol | 30%, 30% activation | Thermoascus aurantiacus | |
propan-2-ol | concentrations below 50%, activation | Thermoascus aurantiacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | - |
Thermoascus aurantiacus | |
D-delta-gluconolactone | - |
Thermoascus aurantiacus | |
ethanol | inhibition above 50% concentration | Thermoascus aurantiacus | |
methanol | inhibition above 50% concentration | Thermoascus aurantiacus | |
propan-2-ol | inhibition above 50% concentration, 50% inhibition at 90%-100% propan-2-ol | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
3 * 120000, SDS-PAGE | Thermoascus aurantiacus |
350000 | - |
gel filtration | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
IMI216529 | - |
Thermoascus aurantiacus IMI216529 | - |
IMI216529 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, Ultrogel AcA 44, Mono P | Thermoascus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
190000 | - |
at pH 4.5 and 60°C | Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-cellobiose + H2O | - |
Thermoascus aurantiacus | 4-methylumbelliferyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-cellobiose + H2O | - |
Thermoascus aurantiacus IMI216529 | 4-methylumbelliferyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | 4-methylumbelliferol + beta-D-glucose | - |
? | |
arbutin + H2O | - |
Thermoascus aurantiacus | beta-D-glucose + ? | - |
? | |
beta-beta-trehalose + H2O | - |
Thermoascus aurantiacus | beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Thermoascus aurantiacus | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Thermoascus aurantiacus IMI216529 | 2 beta-D-glucose | - |
? | |
cellobiose + methanol | 25% methanol, transglycosylation activity | Thermoascus aurantiacus | methyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
CM-cellulose + H2O | - |
Thermoascus aurantiacus | CM-cellulose + beta-D-glucose | - |
? | |
esculin + H2O | - |
Thermoascus aurantiacus | beta-D-glucose + ? | - |
? | |
gentiobiose + H2O | - |
Thermoascus aurantiacus | 2 beta-D-glucose | - |
? | |
gentiobiose + H2O | - |
Thermoascus aurantiacus IMI216529 | 2 beta-D-glucose | - |
? | |
laminarin + H2O | 100times lower activity than with cellobiose | Thermoascus aurantiacus | laminarioligosaccharides + D-glucose | - |
? | |
methyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | methanol + beta-D-glucopyranose | - |
? | |
n-octyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | n-octanol + beta-D-glucose | - |
? | |
o-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | o-nitrophenol + beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | p-nitrophenol + beta-D-glucose | - |
? | |
salicin + H2O | - |
Thermoascus aurantiacus | beta-D-glucose + ? | - |
? | |
salicin + H2O | - |
Thermoascus aurantiacus IMI216529 | beta-D-glucose + ? | - |
? | |
sophorose + H2O | - |
Thermoascus aurantiacus | 2 beta-D-glucose | - |
? | |
sophorose + H2O | - |
Thermoascus aurantiacus IMI216529 | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 120000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermoascus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | 70% of maximal activity at 90°C, low activity below 60°C | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
no loss of activity after 48 h at pH 6.8 | Thermoascus aurantiacus |
70 | - |
no loss of activity after 30 min at pH 5.2 | Thermoascus aurantiacus |
80 | - |
90% loss of activity after 30 min at pH 5.2 | Thermoascus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
136.4 | - |
sophorose | 60°C, pH 4.5 | Thermoascus aurantiacus | |
140.7 | - |
gentiobiose | 60°C, pH 4.5 | Thermoascus aurantiacus | |
234.8 | - |
n-octyl-beta-D-glucopyranoside | 60°C, pH 4.5 | Thermoascus aurantiacus | |
235.2 | - |
o-nitrophenyl-beta-D-glucopyranoside | 60°C, pH 4.5 | Thermoascus aurantiacus | |
242.2 | - |
p-nitrophenyl-beta-D-glucopyranoside | 60°C, pH 4.5 | Thermoascus aurantiacus | |
284.2 | - |
cellobiose | 60°C, pH 4.5 | Thermoascus aurantiacus | |
284.2 | - |
beta,beta-trehalose | 60°C, pH 4.5 | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.8 | 6.8 | approx. 30% of maximal activity at pH 3.5 and pH 6.8, respectively | Thermoascus aurantiacus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
D-delta-gluconolactone | 60°C, pH 4.5 | Thermoascus aurantiacus | |
0.29 | - |
beta-D-glucose | 60°C, pH 4.5 | Thermoascus aurantiacus |