Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | extremely low inhibition | [Candida] sake | |
Hg2+ | 10 mM, 32% inhibition | [Candida] sake | |
Iodine | 1 mM, 69% inhibition | [Candida] sake | |
N-bromosuccinimide | 1 mM, complete inhibition | [Candida] sake |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.9 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, pH 6.0 | [Candida] sake | |
44.6 | - |
cellobiose | 30°C, pH 6.0 | [Candida] sake |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
[Candida] sake | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | [Candida] sake |
220000 | - |
gel filtration | [Candida] sake |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] sake | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephacryl S-300, Q-Sepharose | [Candida] sake |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140.3 | - |
- |
[Candida] sake |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | almost no activity | [Candida] sake | 4-nitrophenol + D-glucose | - |
? | |
arbutin + H2O | best substrate | [Candida] sake | 4-hydroxyphenol + beta-D-glucose | - |
? | |
cellobiose + H2O | weak activity | [Candida] sake | 2 beta-D-glucose | - |
? | |
esculin + H2O | - |
[Candida] sake | esculetin + beta-D-glucose | - |
? | |
o-nitrophenyl-beta-D-glucopyranoside + H2O | - |
[Candida] sake | o-nitrophenol + beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside + H2O | - |
[Candida] sake | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
[Candida] sake | p-nitrophenol + beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-xylopyranoside + H2O | - |
[Candida] sake | p-nitrophenol + beta-D-xylose | - |
? | |
prunasin + H2O | - |
[Candida] sake | D-mandelonitrile + beta-D-glucose | - |
? | |
salicin + H2O | - |
[Candida] sake | salicyl alcohol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, SDS-PAGE | [Candida] sake |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
- |
[Candida] sake |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 65 | approx. 155 of maximal activity at 10°C, approx. 50% of maximal activity at 60°C | [Candida] sake |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
rapid inactivation | [Candida] sake |
55 | - |
complete loss of activity after 4 min | [Candida] sake |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.25 | - |
- |
[Candida] sake |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | approx. 35% of maximal activity at pH 2.5, approx. 50% of maximal activity at pH 6.0 | [Candida] sake |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
beta-D-glucose | 30°C, pH 6.0 | [Candida] sake |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
[Candida] sake | isoelectric focusing | - |
4.9 |