Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | competitive inhibition, Ki: 135 mM | Acetivibrio thermocellus | |
D-glucono-1,5-lactone | competitive inhibition, Ki: 0.01 mM | Acetivibrio thermocellus | |
Hg2+ | complete inactivation at 0.1 mM | Acetivibrio thermocellus | |
iodoacetoamide | complete inhibition at 10 mM | Acetivibrio thermocellus | |
p-chloromercuribenzoic acid | 53% inhibition at 0.5 mM | Acetivibrio thermocellus | |
Tris | 54% inhibition at 10 mM | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Acetivibrio thermocellus | |
83 | - |
cellobiose | - |
Acetivibrio thermocellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | - |
Acetivibrio thermocellus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Acetivibrio thermocellus | low activity | beta-D-glucose + D-glucose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Acetivibrio thermocellus | sophorose | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Acetivibrio thermocellus | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | low activity | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | lactose | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | low activity | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
cellopentaose + 4 H2O | Acetivibrio thermocellus | - |
5 D-glucose | - |
? | |
cellotetraose + 3 H2O | Acetivibrio thermocellus | - |
4 D-glucose | - |
? | |
cellotriose + 2 H2O | Acetivibrio thermocellus | - |
3 D-glucose | - |
? | |
laminaritriose + 2 H2O | Acetivibrio thermocellus | - |
3 D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Acetivibrio thermocellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Acetivibrio thermocellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
127 | - |
- |
Acetivibrio thermocellus |
Storage Stability | Organism |
---|---|
4°C or -20°C without loss of activity for 1 year | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Acetivibrio thermocellus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | low activity | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + beta-D-xylose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | low activity | Acetivibrio thermocellus | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Acetivibrio thermocellus | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
cellobiose + H2O | - |
Acetivibrio thermocellus | 2 beta-D-glucose | - |
? | |
cellopentaose + 4 H2O | - |
Acetivibrio thermocellus | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Acetivibrio thermocellus | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
Acetivibrio thermocellus | 3 D-glucose | - |
? | |
laminaritriose + 2 H2O | - |
Acetivibrio thermocellus | 3 D-glucose | - |
? | |
phenyl-beta-D-glucoside + H2O | low activity | Acetivibrio thermocellus | phenol + beta-D-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
half-life: 62.5 min | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Acetivibrio thermocellus |