Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Ait, N.; Creuzet, N.; Cattaneo, J.
    Properties of beta-glucosidase purified from Clostridium thermocellum (1982), J. Gen. Microbiol., 128, 569-577.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose competitive inhibition, Ki: 135 mM Acetivibrio thermocellus
D-glucono-1,5-lactone competitive inhibition, Ki: 0.01 mM Acetivibrio thermocellus
Hg2+ complete inactivation at 0.1 mM Acetivibrio thermocellus
iodoacetoamide complete inhibition at 10 mM Acetivibrio thermocellus
p-chloromercuribenzoic acid 53% inhibition at 0.5 mM Acetivibrio thermocellus
Tris 54% inhibition at 10 mM Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
4-nitrophenyl-beta-D-glucopyranoside
-
Acetivibrio thermocellus
83
-
cellobiose
-
Acetivibrio thermocellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell associated
-
Acetivibrio thermocellus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acetivibrio thermocellus low activity beta-D-glucose + D-glucose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acetivibrio thermocellus sophorose beta-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acetivibrio thermocellus laminaribiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O Acetivibrio thermocellus low activity beta-D-galactose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O Acetivibrio thermocellus lactose beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Acetivibrio thermocellus cellobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Acetivibrio thermocellus low activity beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Acetivibrio thermocellus gentiobiose beta-D-glucose + D-glucose
-
?
cellopentaose + 4 H2O Acetivibrio thermocellus
-
5 D-glucose
-
?
cellotetraose + 3 H2O Acetivibrio thermocellus
-
4 D-glucose
-
?
cellotriose + 2 H2O Acetivibrio thermocellus
-
3 D-glucose
-
?
laminaritriose + 2 H2O Acetivibrio thermocellus
-
3 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Acetivibrio thermocellus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Acetivibrio thermocellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
127
-
-
Acetivibrio thermocellus

Storage Stability

Storage Stability Organism
4°C or -20°C without loss of activity for 1 year Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Acetivibrio thermocellus 2-hydroxymethylphenol + beta-D-glucose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O low activity Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
4-nitrophenyl beta-D-galactoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + beta-D-xylose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O low activity Acetivibrio thermocellus beta-D-galactose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Acetivibrio thermocellus beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
cellobiose + H2O
-
Acetivibrio thermocellus 2 beta-D-glucose
-
?
cellopentaose + 4 H2O
-
Acetivibrio thermocellus 5 D-glucose
-
?
cellotetraose + 3 H2O
-
Acetivibrio thermocellus 4 D-glucose
-
?
cellotriose + 2 H2O
-
Acetivibrio thermocellus 3 D-glucose
-
?
laminaritriose + 2 H2O
-
Acetivibrio thermocellus 3 D-glucose
-
?
phenyl-beta-D-glucoside + H2O low activity Acetivibrio thermocellus phenol + beta-D-glucose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
68
-
half-life: 62.5 min Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Acetivibrio thermocellus