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Literature summary for 3.2.1.21 extracted from
- Kinetic characterization of a crude beta-D-glucosidase from Aspergillus wentii Pt 2804 (1984), Enzyme Microb. Technol., 6, 508-512.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose | competitive inhibition, Ki: 14 mM | Aspergillus wentii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Aspergillus wentii | |
| 8 | - |
cellobiose | - |
Aspergillus wentii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus wentii | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus wentii | synergistic action of Trichoderma reesei and Aspergillus wentii cellulases for cellulose hydrolysis | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus wentii | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Aspergillus wentii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus wentii | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus wentii | beta-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | synergistic action of Trichoderma reesei and Aspergillus wentii cellulases for cellulose hydrolysis | Aspergillus wentii | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Aspergillus wentii | 2 beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside | Aspergillus wentii |
| 65 | - |
hydrolysis of cellobiose | Aspergillus wentii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 38 h | Aspergillus wentii |
| 65 | - |
half-life: 6.3 h | Aspergillus wentii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5 | hydrolysis of 2-nitrophenyl-beta-D-glucopyranoside | Aspergillus wentii |
| 4.5 | 5.5 | hydrolysis of cellobiose | Aspergillus wentii |
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