Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight activation at 1 mM | Ruminococcus albus | |
Cysteine hydrochloride | slight activation at 1 mM | Ruminococcus albus | |
dithiothreitol | slight activation at 1 mM | Ruminococcus albus | |
GSH | slight activation at 1 mM | Ruminococcus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | remarkably inhibition at 1 mM | Ruminococcus albus | |
Hg2+ | remarkable inhibition at 1 mM | Ruminococcus albus | |
iodoacetoamide | strong inhibition at 1 mM | Ruminococcus albus | |
p-chloromercuribenzoic acid | strong inhibition at 1 mM | Ruminococcus albus | |
Zn2+ | remarkable inhibition at 1 mM | Ruminococcus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Ruminococcus albus | |
26 | - |
cellobiose | - |
Ruminococcus albus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | - |
Ruminococcus albus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
gel filtration, SDS-PAGE | Ruminococcus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Ruminococcus albus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
cellopentaose + 4 H2O | Ruminococcus albus | - |
5 D-glucose | - |
? | |
cellotetraose + 3 H2O | Ruminococcus albus | - |
4 D-glucose | - |
? | |
cellotriose + 2 H2O | Ruminococcus albus | - |
3 D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 12% carbohydrate content | Ruminococcus albus |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Ruminococcus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Ruminococcus albus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
- |
Ruminococcus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-cellobioside + 2 H2O | cleaves beta-glucosidic linkages one by one at the non-reducing end only | Ruminococcus albus | 4-nitrophenol + 2 beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Ruminococcus albus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Ruminococcus albus | beta-D-glucose + D-glucose | - |
? | |
cellobiose + H2O | - |
Ruminococcus albus | 2 beta-D-glucose | - |
? | |
cellopentaose + 4 H2O | - |
Ruminococcus albus | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Ruminococcus albus | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
Ruminococcus albus | 3 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 82000, SDS-PAGE | Ruminococcus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Ruminococcus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable below | Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Ruminococcus albus |