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Literature summary for 3.2.1.21 extracted from

  • Leclerc, M.; Chemardin, P.; Arnaud, A.; Ratomahenina, R.; Galzy, P.; Gerbaud, C.; Raynal, A.; Guerineau, M.
    Comparison of the properties of the purified beta-glucosidase from the transformed strain of Saccharomyces cerevisiae TYKF2 with that of the donor strain Kluyveromyces fragilis Y610 (1987), Biotechnol. Appl. Biochem., 9, 410-422.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
ethanol activation up to 3 mM, above inactivation Kluyveromyces marxianus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Kluyveromyces marxianus

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose competitive inhibition, Ki: 1.6 mM Kluyveromyces marxianus
Hg2+ strong inhibition at 10 mM Kluyveromyces marxianus
Ni2+ strong inhibition at 10 mM Kluyveromyces marxianus
Pb2+ strong inhibition at 10 mM Kluyveromyces marxianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Kluyveromyces marxianus
1.4
-
 Salicin
-
 Kluyveromyces marxianus
5
-
 arbutin
-
 Kluyveromyces marxianus
33
-
 methyl-beta-D-glucoside
-
 Kluyveromyces marxianus
64
-
 cellobiose
-
 Kluyveromyces marxianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 4 * 90000, SDS-PAGE Kluyveromyces marxianus
320000
-
 gel filtration, recombinant and native enzyme Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Kluyveromyces marxianus cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, native and recombinant enzyme Kluyveromyces marxianus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Kluyveromyces marxianus
-
additional information
-
 Kluyveromyces marxianus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.86
-
 native enzyme Kluyveromyces marxianus
64.4
-
 recombinant enzyme Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Kluyveromyces marxianus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Kluyveromyces marxianus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Kluyveromyces marxianus 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Kluyveromyces marxianus 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Kluyveromyces marxianus beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Kluyveromyces marxianus 2 beta-D-glucose
-
 ?
methyl-beta-D-glucoside + H2O
-
 Kluyveromyces marxianus beta-D-glucose + methanol
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 90000, SDS-PAGE Kluyveromyces marxianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Kluyveromyces marxianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Kluyveromyces marxianus