Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Caldicellulosiruptor saccharolyticus |
General Stability | Organism |
---|---|
stable in the presence of cetyl-trimethylammonium bromide | Caldicellulosiruptor saccharolyticus |
stable in the presence of urea | Caldicellulosiruptor saccharolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-O-methyl-beta-D-glucose | competitive inhibition, Ki: 17.5 mM | Caldicellulosiruptor saccharolyticus | |
Co2+ | destabilizing effect | Caldicellulosiruptor saccharolyticus | |
D-glucono-1,4-lactone | Ki: 1.6 mM | Caldicellulosiruptor saccharolyticus | |
D-glucono-1,5-lactone | Ki: 0.09 mM | Caldicellulosiruptor saccharolyticus | |
Hg2+ | strong inactivation at 0.5 mM, reversal by addition of 10 mM beta-mercaptoethanol | Caldicellulosiruptor saccharolyticus | |
La3+ | rapid inactivation at 3 mM | Caldicellulosiruptor saccharolyticus | |
N-ethylmaleimide | rapid loss of activity at 5 mM, reversal by addition of beta-mercaptoethanol | Caldicellulosiruptor saccharolyticus | |
Ni2+ | strong inhibition | Caldicellulosiruptor saccharolyticus | |
SDS | complete inhibition at 1 mg/ml | Caldicellulosiruptor saccharolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
4-nitrophenyl-beta-D-fucoside | - |
Caldicellulosiruptor saccharolyticus | |
0.29 | - |
2-nitrophenyl beta-D-glucopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
0.51 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
1.88 | - |
Salicin | - |
Caldicellulosiruptor saccharolyticus | |
1.94 | - |
4-nitrophenyl-beta-D-galactopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
2.12 | - |
2-nitrophenyl beta-D-galactopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
3 | - |
laminaribiose | - |
Caldicellulosiruptor saccharolyticus | |
5.2 | - |
sophorose | - |
Caldicellulosiruptor saccharolyticus | |
6.93 | - |
4-nitrophenyl-beta-D-xyloside | - |
Caldicellulosiruptor saccharolyticus | |
18.2 | - |
cellobiose | - |
Caldicellulosiruptor saccharolyticus | |
21.5 | - |
gentiobiose | - |
Caldicellulosiruptor saccharolyticus | |
120 | - |
lactose | - |
Caldicellulosiruptor saccharolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Caldicellulosiruptor saccharolyticus | sophorose | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Caldicellulosiruptor saccharolyticus | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | Caldicellulosiruptor saccharolyticus | lactose | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Caldicellulosiruptor saccharolyticus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Caldicellulosiruptor saccharolyticus | gentiobiose | beta-D-glucose + D-glucose | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
tetrahydrofuran | unstable above 20%, v/v | Caldicellulosiruptor saccharolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor saccharolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Caldicellulosiruptor saccharolyticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Caldicellulosiruptor saccharolyticus | - |
additional information | - |
Caldicellulosiruptor saccharolyticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
560 | - |
- |
Caldicellulosiruptor saccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Caldicellulosiruptor saccharolyticus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 2-nitrophenol + beta-D-glucopyranose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
4-nitrophenyl beta-D-fucoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-xylose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Caldicellulosiruptor saccharolyticus | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
cellobiose + H2O | - |
Caldicellulosiruptor saccharolyticus | 2 beta-D-glucose | - |
? | |
gentiobiose + H2O | - |
Caldicellulosiruptor saccharolyticus | 2 beta-D-glucose | - |
? | |
laminaribiose + H2O | - |
Caldicellulosiruptor saccharolyticus | 2 beta-D-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 15 h | Caldicellulosiruptor saccharolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Caldicellulosiruptor saccharolyticus |