Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Wright, R.M.; Yablonsky, M.D.; Shalita, Z.P.; Goyal, A.K.; Eveleigh, D.E.
    Cloning, characterization, and nucleotide sequence of a gene encoding Microbispora bispora BglB, a thermostable beta-glucosidase expressed in Escherichia coli (1992), Appl. Environ. Microbiol., 58, 3455-3465.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
beta-D-glucose cloned enzyme is activated 2-3fold at 0.1-0.3 M Thermobispora bispora

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermobispora bispora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52280
-
 non-denaturating gradient PAGE Thermobispora bispora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermobispora bispora cellobiose beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermobispora bispora cloned enzyme show greater activity against cellobiose than against aryl-beta-D-glucosides beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Thermobispora bispora
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Thermobispora bispora

Source Tissue

Source Tissue Comment Organism Textmining
additional information genomic library Thermobispora bispora
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucoside + H2O
-
 Thermobispora bispora 4-methylumbelliferone + beta-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermobispora bispora beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cloned enzyme show greater activity against cellobiose than against aryl-beta-D-glucosides Thermobispora bispora beta-D-glucose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
BglB
-
 Thermobispora bispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermobispora bispora

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 retaining 70% of its activity after 48 h Thermobispora bispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Thermobispora bispora