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Literature summary for 3.2.1.21 extracted from

  • Pandey, M.; Mishra, S.
    Expression and characterization of Pichia etchellsii beta-glucosidase in Escherichia coli (1997), Gene, 190, 45-51.
    View publication on PubMed
Search for more literature for 3.2.1.21

Application

Application Comment Organism
synthesis synthesis of glycoconjugates and oligosaccharides Schwanniomyces etchellsii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Schwanniomyces etchellsii

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose non-competitive inhibition, Ki: 300 mM Schwanniomyces etchellsii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Schwanniomyces etchellsii
200
-
 cellobiose
-
 Schwanniomyces etchellsii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, SDS-PAGE Schwanniomyces etchellsii
200000
-
 native PAGE Schwanniomyces etchellsii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Schwanniomyces etchellsii sophorose beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucose + H2O Schwanniomyces etchellsii lactose beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Schwanniomyces etchellsii cellobiose beta-D-glucose + D-glucose additional product: cellotetraose as the product of transglycosylation r
6-O-beta-D-glucopyranosyl-D-glucose + H2O Schwanniomyces etchellsii low activity beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Schwanniomyces etchellsii gentiobiose beta-D-glucose + D-glucose
-
 ?
beta-D-fructofuranosyl-alpha-D-glucopyranoside + H2O Schwanniomyces etchellsii
-
 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Schwanniomyces etchellsii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylation irrelevant for enzyme activity Schwanniomyces etchellsii

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Schwanniomyces etchellsii

Source Tissue

Source Tissue Comment Organism Textmining
additional information
-
 Schwanniomyces etchellsii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
-
 Schwanniomyces etchellsii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Schwanniomyces etchellsii 2-hydroxymethylphenol + beta-D-glucose
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Schwanniomyces etchellsii beta-D-glucose + D-glucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O low activity Schwanniomyces etchellsii 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O temperature directed substrate specificity for aryl beta-1,4-linkage Schwanniomyces etchellsii 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Schwanniomyces etchellsii 4-nitrophenol + beta-D-xylose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Schwanniomyces etchellsii beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Schwanniomyces etchellsii beta-D-glucose + D-glucose additional product: cellotetraose as the product of transglycosylation r
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Schwanniomyces etchellsii beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Schwanniomyces etchellsii beta-D-glucose + D-glucose
-
 ?
beta-D-fructofuranosyl-alpha-D-glucopyranoside + H2O
-
 Schwanniomyces etchellsii beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
cellobiose + H2O
-
 Schwanniomyces etchellsii 2 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Schwanniomyces etchellsii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Schwanniomyces etchellsii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 rapid inactivation above Schwanniomyces etchellsii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 for 4-nitrophenyl-beta-D-glucopyranoside Schwanniomyces etchellsii
7
-
 for cellobiose Schwanniomyces etchellsii