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Literature summary for 3.2.1.21 extracted from

  • Kaper, T.; Lebbink, J.H.G.; Pouwels, J.; Kopp, J.; Schulz, G.E.; van der Oost, J.; de Vos, W.M.
    Comparative structural analysis and substrate specificity engineering of the hyperthermostable beta-glucosidase CelB from Pyrococcus furiosus (2000), Biochemistry, 39, 4963-4970.
    View publication on PubMed
Search for more literature for 3.2.1.21

Application

Application Comment Organism
synthesis synthesis of glycoconjugates and oligosaccharides Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
3.3 A resolution Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
E417S mutant with reduced pH optimum, increased Km and turnover number for 2-nitrophenyl-beta-1,4-D-glucopyranoside Pyrococcus furiosus
F426Y mutant with unchanged pH optimum, slight increased Km and slight reduced turnover number for 2-nitrophenyl-beta-1,4-D-glucopyranoside Pyrococcus furiosus
M424K mutant with increased pH optimum, slight increased Km and reduced turnover number for 2-nitrophenyl-beta-1,4-D-galactopyranoside Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 2-Nitrophenyl-beta-D-glucopyranoside
-
 Pyrococcus furiosus
2.3
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Pyrococcus furiosus
31
-
 2-nitrophenyl-beta-D-galactopyranoside-6-phosphate
-
 Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Pyrococcus furiosus

Source Tissue

Source Tissue Comment Organism Textmining
additional information celB gene Pyrococcus furiosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-1,4-D-galactopyranoside 6-phosphate + H2O
-
 Pyrococcus furiosus ?
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + beta-D-galactopyranose
-
 ?
2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O
-
 Pyrococcus furiosus 2-nitrophenol + beta-D-galactopyranose 6-phosphate
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + D-glucopyranose
-
 ?

Subunits

Subunits Comment Organism
tetramer composed of 4 identical subunits, X-ray diffraction study Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
CelB
-
 Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49
-
 2-nitrophenyl beta-1,4-D-galactopyranoside 6-phosphate
-
 Pyrococcus furiosus
860
-
 2-Nitrophenyl-beta-D-glucopyranoside
-
 Pyrococcus furiosus
2050
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 for 2-nitrophenyl-beta-1,4-D-galactopyranoside-6-phosphate Pyrococcus furiosus
5
-
 for 2-nitrophenyl-beta-1,4-D-gluco- and galactopyranoside Pyrococcus furiosus