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Literature summary for 3.2.1.209 extracted from

  • Mora-Montes, H.M.; Robledo-Ortiz, C.I.; Gonzalez-Sanchez, L.C.; Lopez-Esparza, A.; Lopez-Romero, E.; Flores-Carreon, A.
    Purification and biochemical characterisation of endoplasmic reticulum alpha1,2-mannosidase from Sporothrix schenckii (2010), Mem. Inst. Oswaldo Cruz, 105, 79-85.
    View publication on PubMed
Search for more literature for 3.2.1.209

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin the enzyme is inhibited preferentially by 1-deoxymannojirimycin Sporothrix schenckii
Co2+ 50% reduction in alpha-mannosidase activity is observed after addition of 3 mM CoCl2 Sporothrix schenckii
additional information not affected by EDTA Sporothrix schenckii
swainsonine
-
 Sporothrix schenckii
Zn2+ 50% reduction in alpha-mannosidase activity is observed after addition of 3 mM ZnCl2 Sporothrix schenckii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 4-methylumbelliferyl alpha-D-mannopyranoside at pH 7.0 in 50 mM MES-Tris buffer and at 37°C Sporothrix schenckii

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum with the catalytic domain within the lumen of this compartment Sporothrix schenckii 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme activity os not sensitive to the presence of Ca2+, Mg2+ and Mn2+ at concentrations up to 3 mM Sporothrix schenckii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 x * 75000, SDS-PAGE Sporothrix schenckii

Organism

Organism UniProt Comment Textmining
Sporothrix schenckii
-
-
-
Sporothrix schenckii EH206
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Bio-Gel A column chromatography Sporothrix schenckii

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii 4-methylumbelliferone + alpha-D-mannopyranoside
-
 ?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii EH206 4-methylumbelliferone + alpha-D-mannopyranoside
-
 ?
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii 4-nitrophenol + alpha-D-mannopyranoside
-
 ?
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii EH206 4-nitrophenol + alpha-D-mannopyranoside
-
 ?
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
-
 Sporothrix schenckii Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
 ?
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
-
 Sporothrix schenckii EH206 Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
 ?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
-
 Sporothrix schenckii Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
-
 ?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
-
 Sporothrix schenckii EH206 Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Sporothrix schenckii

Synonyms

Synonyms Comment Organism
endoplasmic reticulum alpha1,2-mannosidase
-
 Sporothrix schenckii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.12
-
 at pH 7.0 in 50 mM MES-Tris buffer and at 37°C Sporothrix schenckii 1-deoxymannojirimycin
0.56
-
 at pH 7.0 in 50 mM MES-Tris buffer and at 37°C Sporothrix schenckii swainsonine