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Literature summary for 3.2.1.207 extracted from

  • Torres-Rodriguez, B.I.; Flores-Berrout, K.; Villagomez-Castro, J.C.; Lopez-Romero, E.
    Purification and partial biochemical characterization of a membrane-bound type II-like alpha-glucosidase from the yeast morphotype of Sporothrix schenckii (2012), Antonie van Leeuwenhoek, 101, 313-322.
    View publication on PubMed
Search for more literature for 3.2.1.207

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin more specific inhibitor of alpha-glucosidase II than of alpha-glucosidase I Sporothrix schenckii
castanospermine
-
 Sporothrix schenckii
additional information not inhibited by australine Sporothrix schenckii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 4-Methylumbelliferyl-alpha-D-glucopyranoside in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane 50% of total enzyme activity in a mixed membrane fraction Sporothrix schenckii 16020
-
soluble 38% of total enzyme activity Sporothrix schenckii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75400
-
 2 * 75400, SDS-PAGE Sporothrix schenckii
152700
-
 gel filtration Sporothrix schenckii

Organism

Organism UniProt Comment Textmining
Sporothrix schenckii
-
-
-
Sporothrix schenckii EH206
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography and Sephacryl S-300 gel filtration Sporothrix schenckii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0535
-
 soluble enzyme extract, using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii
0.457
-
 after 8.5fold purification, using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 4 days, 50% loss of activity Sporothrix schenckii
4°C, purified enzyme, 2 days, 50% loss of activity Sporothrix schenckii
4°C, soluble extract, 6 days, 50% loss of activity Sporothrix schenckii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-glucopyranoside + H2O high affinity Sporothrix schenckii 4-methylumbelliferol + alpha-D-glucopyranose
-
 ?
isomaltose + H2O digested at barely detectable level Sporothrix schenckii ?
-
 ?
isomaltose + H2O digested at barely detectable level Sporothrix schenckii EH206 ?
-
 ?
kojibiose + H2O
-
 Sporothrix schenckii ?
-
 ?
kojibiose + H2O
-
 Sporothrix schenckii EH206 ?
-
 ?
maltose + H2O
-
 Sporothrix schenckii ?
-
 ?
maltose + H2O
-
 Sporothrix schenckii EH206 ?
-
 ?
nigerose + H2O preferred substrate over kojibiose, trehalose, and isomaltose (clear preference of the enzyme for the alpha1,3 bond) Sporothrix schenckii ?
-
 ?
nigerose + H2O preferred substrate over kojibiose, trehalose, and isomaltose (clear preference of the enzyme for the alpha1,3 bond) Sporothrix schenckii EH206 ?
-
 ?
trehalose + H2O digested at barely detectable level Sporothrix schenckii ?
-
 ?
trehalose + H2O digested at barely detectable level Sporothrix schenckii EH206 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 75400, SDS-PAGE Sporothrix schenckii

Synonyms

Synonyms Comment Organism
alpha-glucosidase II
-
 Sporothrix schenckii
type II-like alpha-glucosidase
-
 Sporothrix schenckii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Sporothrix schenckii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Sporothrix schenckii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00024
-
 using nigerose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
0.00045
-
 using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
0.00065
-
 using kojibiose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
0.00074
-
 using maltose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
0.00145
-
 using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine
0.0032
-
 using nigerose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine
0.004
-
 using kojibiose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine
0.0068
-
 using maltose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine