Literature summary for 3.2.1.206 extracted from

Guggenheim, K.G.; Crawford, L.M.; Paradisi, F.; Wang, S.C.; Siegel, J.B.
beta-Glucosidase discovery and design for the degradation of oleuropein (2018), ACS Omega, 3, 15754-15762 .

Search for more literature for 3.2.1.206

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces sp.

Protein Variants

Protein Variants	Comment	Organism
C181A	kcat is improved by 3.2fold with the mutant, Km is increased by 4.6fold	Streptomyces sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.86	-	oleuropein	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp.
8.59	-	oleuropein	mutant C181A, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	Q59976	cf. EC 3.2.1.21	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	catalytic mechanism is initiated as residue Glu353 nucleophilically attacks the anomeric carbon of the beta-D-glucosyl residue, displacing the oleuropein aglycone in an SN2 fashion. Glu164 works as an acid/base conduit, protonating the leaving group (oleuropein aglycone) and subsequently deprotonating a water molecule, creating a hydroxide nucleophile that displaces Glu353 and releases beta-D-glucose with retention of stereochemistry. Tyr295 orients the nucleophilic Glu353 to preorganize the active site for attack	Streptomyces sp.	?	-	?
oleuropein + H2O	-	Streptomyces sp.	oleuropein aglycone + D-glucopyranose	-	?

Synonyms

Synonyms	Comment	Organism
Bgl3	-	Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0107	-	oleuropein	mutant C181A, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp.
0.033	-	oleuropein	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0012	-	oleuropein	mutant C181A, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp.
0.0018	-	oleuropein	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp.

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Release 2023.1 (January 2023)