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Literature summary for 3.2.1.206 extracted from

  • Velazquez-Palmero, D.; Romero-Segura, C.; Garcia-Rodriguez, R.; Hernandez, M.L.; Vaistij, F.E.; Graham, I.A.; Perez, A.G.; Martinez-Rivas, J.M.
    An oleuropein beta-glucosidase from olive fruit is involved in determining the phenolic composition of virgin olive oil (2017), Front. Plant Sci., 8, 1902 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.206

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana benthamiana leaves Olea europaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oleuropein + H2O Olea europaea
-
 oleuropein aglycone + D-glucopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Olea europaea A0A2H4ER88 cultivars Picual and Arbequina
-

Purification (Commentary)

Purification (Comment) Organism
His GraviTrap column chromatography Olea europaea

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Olea europaea
-
mesocarp
-
 Olea europaea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
demethyloleuropein + H2O 15.6% activity compared to oleuropein Olea europaea ?
-
 ?
ligstroside + H2O 25.6% activity compared to oleuropein Olea europaea ligstroside aglycone + D-glucopyranose
-
 ?
oleuropein + H2O
-
 Olea europaea oleuropein aglycone + D-glucopyranose
-
 ?
oleuropein + H2O 100% activity Olea europaea oleuropein aglycone + D-glucopyranose
-
 ?

Subunits

Subunits Comment Organism
? x * 62600, calculated from amino acid sequence Olea europaea
? x * 65500, SDS-PAGE Olea europaea

Synonyms

Synonyms Comment Organism
GLU
-
 Olea europaea
oleuropein beta-glucosidase
-
 Olea europaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Olea europaea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 more than 75% activity between 25 and 45°C Olea europaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Olea europaea

pH Range

pH Minimum pH Maximum Comment Organism
4 6 more than 50% activity between pH 4.0 and 6.0 Olea europaea

pI Value

Organism Comment pI Value Maximum pI Value
Olea europaea calculated from amino acid sequence
-
 6.6

Expression

Organism Comment Expression
Olea europaea enzyme expression is decreased at darkness (24h) and after wounding down
Olea europaea enzyme expression is increased at low temperature (15°C) up