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Literature summary for 3.2.1.204 extracted from

  • Tagami, T.; Miyano, E.; Sadahiro, J.; Okuyama, M.; Iwasaki, T.; Kimura, A.
    Two novel glycoside hydrolases responsible for the catabolism of cyclobis-(1->6)-alpha-nigerosyl (2016), J. Biol. Chem., 291, 16438-16447 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.204

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Kribbella flavida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.63
-
 cyclobis-(1->6)-alpha-nigerosyl pH 8.0, 35°C Kribbella flavida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclobis-(1->6)-alpha-nigerosyl + 2 H2O Kribbella flavida
-
 2 isomaltose
-
 ?
cyclobis-(1->6)-alpha-nigerosyl + 2 H2O Kribbella flavida DSM 17836
-
 2 isomaltose
-
 ?
additional information Kribbella flavida the enzyme hydrolyzes the alpha-(1->3)-glucosidic linkages of cyclobis-(1->6)-nigerosyl and produces isomaltose via a possible linear tetrasaccharide. It has a strong preference for the alpha-(1->3)-isomaltosyl moiety and effectively hydrolyzes the alpha-(1->3)-glucosidic linkage ?
-
 ?
additional information Kribbella flavida DSM 17836 the enzyme hydrolyzes the alpha-(1->3)-glucosidic linkages of cyclobis-(1->6)-nigerosyl and produces isomaltose via a possible linear tetrasaccharide. It has a strong preference for the alpha-(1->3)-isomaltosyl moiety and effectively hydrolyzes the alpha-(1->3)-glucosidic linkage ?
-
 ?

Organism

Organism UniProt Comment Textmining
Kribbella flavida D2PPM7 glycoside hydrolase family 31 protein
-
Kribbella flavida DSM 17836 D2PPM7 glycoside hydrolase family 31 protein
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobis-(1->6)-alpha-nigerosyl + 2 H2O
-
 Kribbella flavida 2 isomaltose
-
 ?
cyclobis-(1->6)-alpha-nigerosyl + 2 H2O
-
 Kribbella flavida DSM 17836 2 isomaltose
-
 ?
additional information the enzyme hydrolyzes the alpha-(1->3)-glucosidic linkages of cyclobis-(1->6)-nigerosyl and produces isomaltose via a possible linear tetrasaccharide. It has a strong preference for the alpha-(1->3)-isomaltosyl moiety and effectively hydrolyzes the alpha-(1->3)-glucosidic linkage Kribbella flavida ?
-
 ?
additional information the enzyme hydrolyzes the alpha-(1->3)-glucosidic linkages of cyclobis-(1->6)-nigerosyl and produces isomaltose via a possible linear tetrasaccharide. It has a strong preference for the alpha-(1->3)-isomaltosyl moiety and effectively hydrolyzes the alpha-(1->3)-glucosidic linkage Kribbella flavida DSM 17836 ?
-
 ?
panose + H2O
-
 Kribbella flavida isomaltose + alpha-D-glucopyranose
-
 ?
panose + H2O
-
 Kribbella flavida DSM 17836 isomaltose + alpha-D-glucopyranose
-
 ?

Synonyms

Synonyms Comment Organism
Kfla1895
-
 Kribbella flavida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
41
-
 stable below Kribbella flavida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.3
-
 cyclobis-(1->6)-alpha-nigerosyl pH 8.0, 35°C Kribbella flavida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.8 10.7
-
 Kribbella flavida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.052
-
 panose pH 8.0, 35°C Kribbella flavida