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Literature summary for 3.2.1.203 extracted from

  • Duman, Y.; Yuzugullu Karakus, Y.; Sertel, A.; Polat, F.
    Production, purification, and characterization of a thermo-alkali stable and metal-tolerant carboxymethylcellulase from newly isolated Bacillus methylotrophicus Y37 (2016), Turk. J. Chem., 40, 802-815 .
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
N-bromosuccinimide 114.27% activity at 10 mM Bacillus velezensis
TPCK 121.32% activity at 10 mM Bacillus velezensis

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid 30.33% residual activity at 10 mM Bacillus velezensis
phenyl glyoxylate 75.06% residual activity at 10 mM Bacillus velezensis
phenylmethylsulfonyl fluoride 35.54% residual activity at 10 mM Bacillus velezensis
TLCK 72.15% residual activity at 10 mM Bacillus velezensis
Woodward's reagent K 56.98% residual activity at 10 mM Bacillus velezensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 114.2% activity at 100 mM Bacillus velezensis
Cd2+ 114.2% activity at 100 mM Bacillus velezensis
Co2+ 319.1% activity at 100 mM Bacillus velezensis
K+ 148.4% activity at 100 mM Bacillus velezensis
Mg2+ 141.2% activity at 100 mM Bacillus velezensis
Na+ 103.1% activity at 100 mM Bacillus velezensis

Organism

Organism UniProt Comment Textmining
Bacillus velezensis
-
-
-
Bacillus velezensis Y37
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography Bacillus velezensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.39
-
 using (carboxymethyl)cellulose as substrate, at pH 7.0 and 50°C Bacillus velezensis
13.13
-
 using filter paper as substrate, at pH 7.0 and 50°C Bacillus velezensis
14.19
-
 using laminarin as substrate, at pH 7.0 and 50°C Bacillus velezensis
14.89
-
 using glucan as substrate, at pH 7.0 and 50°C Bacillus velezensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(carboxymethyl)cellulose + H2O
-
 Bacillus velezensis ?
-
 ?
(carboxymethyl)cellulose + H2O
-
 Bacillus velezensis Y37 ?
-
 ?
filter paper + H2O
-
 Bacillus velezensis ?
-
 ?
filter paper + H2O
-
 Bacillus velezensis Y37 ?
-
 ?
glucan + H2O highest activity Bacillus velezensis ?
-
 ?
glucan + H2O highest activity Bacillus velezensis Y37 ?
-
 ?
laminarin + H2O
-
 Bacillus velezensis ?
-
 ?
laminarin + H2O
-
 Bacillus velezensis Y37 ?
-
 ?
additional information no activity with 2-nitrophenyl beta -D-galactopyranoside Bacillus velezensis ?
-
 ?
additional information no activity with 2-nitrophenyl beta -D-galactopyranoside Bacillus velezensis Y37 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Bacillus velezensis

Synonyms

Synonyms Comment Organism
CMCase
-
 Bacillus velezensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus velezensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 more than 90% of the original enzyme activity is maintained at temperatures ranging from 55 to 65°C after 45 min. Above 65°C, the enzyme activity is moderately lost with 65% remaining Bacillus velezensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus velezensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 after incubation at 4°C and pH 10 for 3 h the enzyme retains 82% of the original activity, while this is 74% for incubation at 50°C and pH 10 for 1 h Bacillus velezensis