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Literature summary for 3.2.1.200 extracted from

  • Laribi-Habchi, H.; Bouanane-Darenfed, A.; Drouiche, N.; Pauss, A.; Mameri, N.
    Purification, characterization, and molecular cloning of an extracellular chitinase from Bacillus licheniformis stain LHH100 isolated from wastewater samples in Algeria (2015), Int. J. Biol. Macromol., 72, 1117-1128 .
    View publication on PubMed
Search for more literature for 3.2.1.200

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 5 mM, 35% residual activity Bacillus licheniformis
dithiothreitol 2 mM, 10% residual activity Bacillus licheniformis
Hg2+ 5 mM, complete inhibition Bacillus licheniformis
N-ethylmaleimide 2 mM, complete inhibition Bacillus licheniformis
p-chloromercuribenzoate 2 mM, complete inhibition Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 colloidal chitin Km value 0.385 mg/ml, pH 4.0, 70°C Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 5 mM, 113% of initial activity Bacillus licheniformis
K+ 5 mM, 145% of initial activity Bacillus licheniformis
Li+ 5 mM, 132% of initial activity Bacillus licheniformis
Zn2+ 5 mM, 131% of initial activity Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65193
-
 MALDI-TOF Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis A0A0G2QUN1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-N,N',N'', N'''-tetraacetylchitotetraoside + H2O
-
 Bacillus licheniformis ?
-
 ?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Bacillus licheniformis ?
-
 ?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Bacillus licheniformis ?
-
 ?
chitooligosaccharide + H2O
-
 Bacillus licheniformis ?
-
 ?
chitosan + H2O
-
 Bacillus licheniformis N,N'-diacetylchitobiose + ?
-
 ?
chitotriose + H2O
-
 Bacillus licheniformis ?
-
 ?
colloidal chitin + H2O
-
 Bacillus licheniformis N,N'-diacetylchitobiose + ?
-
 ?
glycol chitin + H2O
-
 Bacillus licheniformis N,N'-diacetylchitobiose + ?
-
 ?
additional information the enzmye preferentially hydrolyzes the second glycosidic link from the non-reducing end of (GlcNAc)n Bacillus licheniformis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65193, MALDI-TOF Bacillus licheniformis

Synonyms

Synonyms Comment Organism
ChiA 65
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5000
-
 colloidal chitin pH 4.0, 70°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Bacillus licheniformis