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Literature summary for 3.2.1.20 extracted from

  • Chen, P.; Xu, R.; Wang, J.; Wu, Z.; Yan, L.; Zhao, W.; Liu, Y.; Ma, W.; Shi, X.; Li, H.
    Starch biotransformation into isomaltooligosaccharides using thermostable alpha-glucosidase from Geobacillus stearothermophilus (2018), PeerJ, 6, e5086 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the alpha-transglucosidase-producing Geobacillus stearothermophilus as a potential application technique can be successfully used to prepare industrial isomaltooligosaccharides (IMOs) Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
additional information biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2. 5 l and 20 l scale fermentations are performed. After isolation and purification, liquid alpha-glucosidase preparations are obtained. Through covalent cross-linking and adsorption cross-linking using magnetic chitosan microspheres as the carrier and glutaraldehyde as the crosslinking agent, the conditions for immobilization of alpha-glucosidase on chitosan are determined. Isomaltooligosaccharides (IMOs) are prepared using chitosan membrane-immobilized alpha-glucosidase, beta-amylase, pullulanase, fungal alpha-amylase and starch as substrate. The mixed syrup that contains IMOs is evaluated and analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). In addition, small-scale preparation of IMOs is performed. Method, overview Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ slight inhibition Geobacillus stearothermophilus
Cu2+ strong inhibition Geobacillus stearothermophilus
Fe2+ moderate inhibition Geobacillus stearothermophilus
Zn2+ moderate inhibition Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Geobacillus stearothermophilus
K+ activates Geobacillus stearothermophilus
Mg2+ activates Geobacillus stearothermophilus
Mn2+ activates Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltose + H2O Geobacillus stearothermophilus
-
alpha-D-glucose + D-glucose
-
?
maltose + H2O Geobacillus stearothermophilus U2
-
alpha-D-glucose + D-glucose
-
?
additional information Geobacillus stearothermophilus biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 ?
-
?
additional information Geobacillus stearothermophilus U2 biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 ?
-
?
starch + H2O Geobacillus stearothermophilus
-
?
-
?
starch + H2O Geobacillus stearothermophilus
-
alpha-D-glucose + ?
-
?
starch + H2O Geobacillus stearothermophilus U2
-
alpha-D-glucose + ?
-
?
starch + H2O Geobacillus stearothermophilus U2
-
?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus Q9R5L9 i.e. Bacillus stearothermophilus
-
Geobacillus stearothermophilus U2
-
-
-
Geobacillus stearothermophilus U2 Q9R5L9 i.e. Bacillus stearothermophilus
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of culture conditions for wild-type and engineered strains, overview Geobacillus stearothermophilus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Geobacillus stearothermophilus 4-nitrophenol + D-glucose
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Geobacillus stearothermophilus U2 4-nitrophenol + D-glucose
-
?
4-nitrophenyl alpha-D-glucoside + H2O
-
Geobacillus stearothermophilus 4-nitrophenol + alpha-D-glucose
-
?
4-nitrophenyl alpha-D-glucoside + H2O
-
Geobacillus stearothermophilus U2 4-nitrophenol + alpha-D-glucose
-
?
maltose + H2O
-
Geobacillus stearothermophilus alpha-D-glucose + D-glucose
-
?
maltose + H2O
-
Geobacillus stearothermophilus U2 alpha-D-glucose + D-glucose
-
?
additional information biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 Geobacillus stearothermophilus ?
-
?
additional information biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 Geobacillus stearothermophilus U2 ?
-
?
starch + H2O
-
Geobacillus stearothermophilus ?
-
?
starch + H2O
-
Geobacillus stearothermophilus U2 ?
-
?
starch + H2O
-
Geobacillus stearothermophilus alpha-D-glucose + ?
-
?
starch + H2O
-
Geobacillus stearothermophilus U2 alpha-D-glucose + ?
-
?

Synonyms

Synonyms Comment Organism
alpha-transglucosidase
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
free enzyme Geobacillus stearothermophilus
50
-
enzyme immobilized on magnetic chitosan microspheres Geobacillus stearothermophilus
60
-
-
Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 the free enzyme is active between 35 and 55°C Geobacillus stearothermophilus
40 60 the enzyme immobilized on magnetic chitosan microspheres shows about 40% activity at 30°C, about 50% activity at 40°C, 100% activity at 50°C, and about 90% activity at 60°C Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 80 the enzyme is thermostable for 24 h and no loss in activity is found below 37°C, but it is inactivated at 65°C or more and completely loses its activity at 80°C Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.4 the enzyme is active between pH 6.2 and 7.4 Geobacillus stearothermophilus