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Literature summary for 3.2.1.20 extracted from

  • Cuebas-Irizarry, M.F.; Irizarry-Caro, R.A.; Lopez-Morales, C.; Badillo-Rivera, K.M.; Rodriguez-Minguela, C.M.; Montalvo-Rodriguez, R.
    Cloning and molecular characterization of an alpha-glucosidase (MalH) from the halophilic archaeon Haloquadratum walsbyi (2017), Life, 7, E46 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Haloquadratum walsbyi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Haloquadratum walsbyi 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ enzyme activity is the highest in the presence of 3-4 M KCl Haloquadratum walsbyi
Na+ enzyme activity is the highest in the presence of 3 M NaCl Haloquadratum walsbyi

Organism

Organism UniProt Comment Textmining
Haloquadratum walsbyi
-
-
-
Haloquadratum walsbyi DSM 16854
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Haloquadratum walsbyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Haloquadratum walsbyi 4-nitrophenol + alpha-D-glucopyranose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Haloquadratum walsbyi DSM 16854 4-nitrophenol + alpha-D-glucopyranose
-
 ?
additional information no activity with 4-nitrophenyl alpha-D-xylopyranoside Haloquadratum walsbyi ?
-
 ?
additional information no activity with 4-nitrophenyl alpha-D-xylopyranoside Haloquadratum walsbyi DSM 16854 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 87000, SDS-PAGE Haloquadratum walsbyi

Synonyms

Synonyms Comment Organism
MalH
-
 Haloquadratum walsbyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Haloquadratum walsbyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Haloquadratum walsbyi