Cloned (Comment) | Organism |
---|---|
gene aglU, recombinant expression from plasmid pKANGL-GCW61 in Pichia pastoris strain GS115, cell surface display, recombinant expression in Escherichia coli TOP10 cells | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
additional information | preparation of the AglU-displaying Pichia pastoris whole-cell biocatalyst for optimized synthesis of isomaltooligosaccharides (IMOs). IMOs are a mixture that includes isomaltose (IG2), panose (P), and isomaltotriose (IG3). The recombinant Aspergillus niger enzyme expressed from Pichia pastoris has a high reaction efficiency and operational stability, method optimization and evaluation, overview. IMOs synthesis by transglycosylation, best at pH 4.0 and 55-60°C | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus niger | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A2QJF7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Aspergillus niger | 2 D-glucose | - |
? | |
additional information | the recombinant enzyme performs isomaltooligosaccharides (IMOs) synthesis by transglycosylation. Quantitative analyses of products maltose (M), isomaltose (IG2), panose (P), and isomaltotriose (IG3) | Aspergillus niger | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aGlu | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Aspergillus niger |