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Literature summary for 3.2.1.20 extracted from

  • Kaewmuangmoon, J.; Kilaso, M.; Leartsakulpanich, U.; Kimura, K.; Kimura, A.; Chanchao, C.
    Expression of a secretory alpha-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization (2013), BMC Biotechnol., 13, 16.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene AciHBGase II, DNA and amino acid sequence determination and analysis, recombinant expression of the C-terminally His-tagged secretory enzyme in Pichia pastoris strain GS115 Apis cerana indica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Apis cerana indica
4.2
-
4-nitrophenyl alpha-D-glucoside recombinant enzyme, pH 5.5, 37°C Apis cerana indica
15.3
-
sucrose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
22
-
soluble starch recombinant enzyme, pH 5.5, 37°C Apis cerana indica
22.3
-
isomaltose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
25
-
maltotetraose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
26.7
-
maltotriose recombinant enzyme, pH 5.5, 37°C Apis cerana indica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretory enzyme Apis cerana indica
-
-

Organism

Organism UniProt Comment Textmining
Apis cerana indica J9SJ08 gene AciHBGase II
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular His-tagged enzyme 1.45fold from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, nickel affinity chromatography to homogeneity Apis cerana indica

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Apis cerana indica
-
additional information highest transcript expression level of the isozyme is found in larvae and pupae, with lower levels in the eggs, no activity in foragers, comparison of expression patterns to isozyme I and III and to isozymes from Apis mellifera, overview Apis cerana indica
-
pupa
-
Apis cerana indica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
purified recombinant His-tagged enzyme, pH 5.5, 37°C, substrate 4-nitrophenyl alpha-D-glucoside Apis cerana indica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucoside + H2O low activity Apis cerana indica 4-nitrophenol + D-glucose
-
?
isomaltose + H2O low activity Apis cerana indica ?
-
?
maltose + H2O low activity Apis cerana indica ?
-
?
maltotetraose + H2O low activity Apis cerana indica ?
-
?
maltotriose + H2O low activity Apis cerana indica ?
-
?
additional information the enzyme shows a wide substrate specificity cleaving alpha-(1,1)-, alpha-(1,2)-, alpha-(1,4)- and alpha-(1,6)-glucosidic linkages, and rapidly hydrolyzing 4-nitrophenyl alpha-D-glucoside alpha-(1,6) and sucrose alpha-(1,2) linkages. Recombinant and native display positive cooperativity to sucrose, turanose alpha-(1,3), kojibiose alpha-(1,2) and soluble starch alpha-(1,4), the recombinant His-tagged enzyme hydrolyzes isomaltose and soluble starch but with different Km values Apis cerana indica ?
-
?
soluble starch + H2O low activity Apis cerana indica ?
-
?
sucrose + H2O highly preferred substrate Apis cerana indica ?
-
?

Synonyms

Synonyms Comment Organism
alpha-glucosidase II
-
Apis cerana indica
HBGase II
-
Apis cerana indica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
recombinant His-tagged enzyme Apis cerana indica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 recombinant His-tagged enzyme, over 80% of maximal activity in this range Apis cerana indica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 65 purified recombinant His-tagged enzyme, over 80% activity remaining Apis cerana indica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.89
-
4-nitrophenyl alpha-D-glucoside recombinant enzyme, pH 5.5, 37°C Apis cerana indica
2.84
-
maltotetraose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
4.6
-
soluble starch recombinant enzyme, pH 5.5, 37°C Apis cerana indica
5.2
-
isomaltose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
26.6
-
maltotriose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
845
-
sucrose recombinant enzyme, pH 5.5, 37°C Apis cerana indica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
recombinant His-tagged enzyme Apis cerana indica

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5 recombinant His-tagged enzyme, over 80% of maximal activity in this range Apis cerana indica

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 purified recombinant His-tagged enzyme, over 80% activity remaining Apis cerana indica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
maltotetraose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
0.21
-
soluble starch recombinant enzyme, pH 5.5, 37°C Apis cerana indica
0.23
-
isomaltose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
0.45
-
4-nitrophenyl alpha-D-glucoside recombinant enzyme, pH 5.5, 37°C Apis cerana indica
1
-
maltotriose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
55.4
-
sucrose recombinant enzyme, pH 5.5, 37°C Apis cerana indica