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Literature summary for 3.2.1.20 extracted from

  • Golyshina, O.V.; Golyshin, P.N.; Timmis, K.N.; Ferrer, M.
    The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum (2006), Environ. Microbiol., 8, 416-425.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ferroplasma acidiphilum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Ferroplasma acidiphilum 5622
-
membrane
-
Ferroplasma acidiphilum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28316
-
1 * 28316, calculated from sequence Ferroplasma acidiphilum
40505
-
1 * 40505, calculated from sequence Ferroplasma acidiphilum
42000
-
1 * 42000, SDS-PAGE Ferroplasma acidiphilum
56000
-
non-denasturing PAGE Ferroplasma acidiphilum
57000
-
non-denaturing PAGE Ferroplasma acidiphilum
57300
-
1 * 57300, calculated from sequence Ferroplasma acidiphilum
80000
-
non-denasturing PAGE Ferroplasma acidiphilum

Organism

Organism UniProt Comment Textmining
Ferroplasma acidiphilum Q2PCE2
-
-
Ferroplasma acidiphilum Q2PCE3
-
-
Ferroplasma acidiphilum Q5K3Q3
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from both Escherichia coli and Ferroplasma acidiphilum Ferroplasma acidiphilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
Ferroplasma acidiphilum 2 D-glucose
-
?
maltotetraose + H2O very low activity Ferroplasma acidiphilum ?
-
?
maltotriose + H2O
-
Ferroplasma acidiphilum ?
-
?
sucrose + H2O
-
Ferroplasma acidiphilum D-glucose + D-fructose
-
?

Subunits

Subunits Comment Organism
? 1 * 42000, SDS-PAGE Ferroplasma acidiphilum
? 1 * 28316, calculated from sequence Ferroplasma acidiphilum
? 1 * 40505, calculated from sequence Ferroplasma acidiphilum
? 1 *28000, SDS-PAGE Ferroplasma acidiphilum
monomer 1 * 57000, SDS-PAGE Ferroplasma acidiphilum
monomer 1 * 57300, calculated from sequence Ferroplasma acidiphilum

Synonyms

Synonyms Comment Organism
alphaGluFa
-
Ferroplasma acidiphilum
GlyFa1
-
Ferroplasma acidiphilum
GlyFa2
-
Ferroplasma acidiphilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Ferroplasma acidiphilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 1.7-2.5, half-life: 23 min Ferroplasma acidiphilum
60
-
pH 2.5-3.5, half-life: 34 min Ferroplasma acidiphilum
60
-
pH 3.5-4.0, half-life: 12 min Ferroplasma acidiphilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
-
Ferroplasma acidiphilum
2.5 3
-
Ferroplasma acidiphilum
3.5 4
-
Ferroplasma acidiphilum

pH Range

pH Minimum pH Maximum Comment Organism
1 4 pH 1.0: about 45% of maximal activity, pH 4.0: about 55% of maximal activity Ferroplasma acidiphilum
1.5 5 pH 1.5: about 70% of maximal activity, pH 5.0: about 60% of maximal activity Ferroplasma acidiphilum
2.5 5.5 pH 2.5: about 40% of maximal activity, pH 5.5: about 40% of maximal activity Ferroplasma acidiphilum

pI Value

Organism Comment pI Value Maximum pI Value
Ferroplasma acidiphilum calculated from sequence
-
6.42
Ferroplasma acidiphilum calculated from sequence
-
9.53

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
maltotetraose 60°C, pH not specified in the publication Ferroplasma acidiphilum
0.3
-
maltotetraose 60°C, pH not specified in the publication Ferroplasma acidiphilum
0.6
-
maltotetraose 60°C, pH not specified in the publication Ferroplasma acidiphilum
18.8
-
maltotriose 60°C, pH not specified in the publication Ferroplasma acidiphilum
28
-
maltotriose 60°C, pH not specified in the publication Ferroplasma acidiphilum
37
-
maltotriose 60°C, pH not specified in the publication Ferroplasma acidiphilum
73
-
maltose 60°C, pH not specified in the publication Ferroplasma acidiphilum
101
-
maltose 60°C, pH not specified in the publication Ferroplasma acidiphilum
142
-
sucrose 60°C, pH not specified in the publication Ferroplasma acidiphilum
197
-
maltose 60°C, pH not specified in the publication Ferroplasma acidiphilum
293
-
sucrose 60°C, pH not specified in the publication Ferroplasma acidiphilum