Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ferroplasma acidiphilum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Ferroplasma acidiphilum | 5622 | - |
membrane | - |
Ferroplasma acidiphilum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28316 | - |
1 * 28316, calculated from sequence | Ferroplasma acidiphilum |
40505 | - |
1 * 40505, calculated from sequence | Ferroplasma acidiphilum |
42000 | - |
1 * 42000, SDS-PAGE | Ferroplasma acidiphilum |
56000 | - |
non-denasturing PAGE | Ferroplasma acidiphilum |
57000 | - |
non-denaturing PAGE | Ferroplasma acidiphilum |
57300 | - |
1 * 57300, calculated from sequence | Ferroplasma acidiphilum |
80000 | - |
non-denasturing PAGE | Ferroplasma acidiphilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ferroplasma acidiphilum | Q2PCE2 | - |
- |
Ferroplasma acidiphilum | Q2PCE3 | - |
- |
Ferroplasma acidiphilum | Q5K3Q3 | - |
- |
Purification (Comment) | Organism |
---|---|
purified from both Escherichia coli and Ferroplasma acidiphilum | Ferroplasma acidiphilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Ferroplasma acidiphilum | 2 D-glucose | - |
? | |
maltotetraose + H2O | very low activity | Ferroplasma acidiphilum | ? | - |
? | |
maltotriose + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
sucrose + H2O | - |
Ferroplasma acidiphilum | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 42000, SDS-PAGE | Ferroplasma acidiphilum |
? | 1 * 28316, calculated from sequence | Ferroplasma acidiphilum |
? | 1 * 40505, calculated from sequence | Ferroplasma acidiphilum |
? | 1 *28000, SDS-PAGE | Ferroplasma acidiphilum |
monomer | 1 * 57000, SDS-PAGE | Ferroplasma acidiphilum |
monomer | 1 * 57300, calculated from sequence | Ferroplasma acidiphilum |
Synonyms | Comment | Organism |
---|---|---|
alphaGluFa | - |
Ferroplasma acidiphilum |
GlyFa1 | - |
Ferroplasma acidiphilum |
GlyFa2 | - |
Ferroplasma acidiphilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Ferroplasma acidiphilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 1.7-2.5, half-life: 23 min | Ferroplasma acidiphilum |
60 | - |
pH 2.5-3.5, half-life: 34 min | Ferroplasma acidiphilum |
60 | - |
pH 3.5-4.0, half-life: 12 min | Ferroplasma acidiphilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Ferroplasma acidiphilum |
2.5 | 3 | - |
Ferroplasma acidiphilum |
3.5 | 4 | - |
Ferroplasma acidiphilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 4 | pH 1.0: about 45% of maximal activity, pH 4.0: about 55% of maximal activity | Ferroplasma acidiphilum |
1.5 | 5 | pH 1.5: about 70% of maximal activity, pH 5.0: about 60% of maximal activity | Ferroplasma acidiphilum |
2.5 | 5.5 | pH 2.5: about 40% of maximal activity, pH 5.5: about 40% of maximal activity | Ferroplasma acidiphilum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ferroplasma acidiphilum | calculated from sequence | - |
6.42 |
Ferroplasma acidiphilum | calculated from sequence | - |
9.53 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
maltotetraose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
0.3 | - |
maltotetraose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
0.6 | - |
maltotetraose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
18.8 | - |
maltotriose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
28 | - |
maltotriose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
37 | - |
maltotriose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
73 | - |
maltose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
101 | - |
maltose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
142 | - |
sucrose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
197 | - |
maltose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
293 | - |
sucrose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum |