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Literature summary for 3.2.1.20 extracted from

  • Nawaz, M.; Karim, A.; Aman, A.; Marchetti, R.; Qader, S.; Molinaro, A.
    Continuous degradation of maltose: improvement in stability and catalytic properties of maltase (alpha-glucosidase) through immobilization using agar-agar gel as a support (2015), Bioprocess Biosyst. Eng., 38, 631-638.
    View publication on PubMed
Search for more literature for 3.2.1.20

Application

Application Comment Organism
synthesis immobilization of enzyme within agar-agar support via entrapment. The maximum immobilization efficiency of 82.77% is achieved using 4.0% agar-agar keeping the diameter of beads up to 3.0 mm. Km value of immobilized enzyme increases from 1.717 to 2.117 mM whereas Vmax decreases from 8,411 to 7,450 U/min as compared to free enzyme. The immobilization significantly increases the stability of maltase against various temperatures and immobilized maltase retains 100% of its original activity after 2 h at 50°C, whereas the free maltase only shows 60% residual activity under the same conditions. Entrapped maltase can be reused for up to 12 cycles and retains 50% of activity even after the 5th cycle. Agar entrapped maltase retains 73% of its initial activity even after 2 months when stored at 30°C Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.72
-
 maltose free enzyme, pH not specified in the publication, temperature not specified in the publication Bacillus licheniformis
2.12
-
 maltose immobilized enzyme entrapped in agar, pH not specified in the publication, temperature not specified in the publication Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus licheniformis KIBGE-IB4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
 Bacillus licheniformis 2 D-glucose
-
 ?
maltose + H2O
-
 Bacillus licheniformis KIBGE-IB4 2 D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 immobilized enzyme entrapped in agar Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 immobilized maltase retains 100% of its original activity after 2 h at 50°C, whereas the free maltase shows 60% residual activity Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 immobilized enzyme entrapped in agar Bacillus licheniformis