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Literature summary for 3.2.1.20 extracted from
- Identification and characterization of MalA in the maltose/maltodextrin operon of Sulfolobus acidocaldarius DSM639 (2013), J. Bacteriol., 195, 1789-1799.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sulfolobus acidocaldarius. Attempts to obtain a soluble enzyme from Escherichia coli strains are unsuccessful | Sulfolobus acidocaldarius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, pH 5.0, 95°C, 24% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius |  |
| Cu2+ | 1 mM, pH 5.0, 95°C, 91% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
| EDTA | 1 mM, pH 5.0, 95°C, 11% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
| Fe2+ | 1 mM, pH 5.0, 95°C, 39% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
| SDS | 1%, pH 5.0, 95°C, complete inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
| Urea | 1 mM, pH 5.0, 95°C, 26% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
| Zn2+ | 1 mM, pH 5.0, 95°C, complete inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
maltotriose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 0.3 | - |
maltotetraose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 0.32 | - |
maltopentaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 0.52 | - |
maltohexaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 0.8 | - |
maltose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 0.87 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Sulfolobus acidocaldarius | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM, pH 5.0, 95°C, 1.7fold activation of hydrolysis of 4-nitrophenyl alpha-glucopyranoside | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
| 73000 | - |
12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
| 914000 | - |
gel filtration | Sulfolobus acidocaldarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | Sulfolobus acidocaldarius | the enzyme is involved in maltose and starch metabolism | alpha-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | Q4J9M3 | - |
- |
| Sulfolobus acidocaldarius DSM 639 | Q4J9M3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:maltose-grown cell | the transcription level of malA is increased 3-fold upon the addition of maltose to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11-fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars | Sulfolobus acidocaldarius | - |
| culture condition:starch-grown cell | the transcription level of malA is increased 3-fold upon the addition of starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 10-fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, respectively, than during growth on other sugars | Sulfolobus acidocaldarius | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stored for at least 6 months without any loss of activity | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Sulfolobus acidocaldarius | 4-nitrophenol + alpha-D-glucose | - |
? | |
| isomaltose + H2O | i.e. alpha-D-Glc-(1->6)-D-Glc | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
| kojibiose + H2O | i.e. alpha-D-Glc-(1->2)-D-Glc | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
| kojibiose + H2O | i.e. alpha-D-Glc-(1->2)-D-Glc | Sulfolobus acidocaldarius DSM 639 | alpha-D-glucose + D-glucose | - |
? | |
| maltohexaose + H2O | - |
Sulfolobus acidocaldarius | maltopentaose + D-glucose | - |
? | |
| maltopentaose + H2O | - |
Sulfolobus acidocaldarius | alpha-D-glucose + ? | - |
? | |
| maltose + H2O | - |
Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | the enzyme is involved in maltose and starch metabolism | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
| maltotetraose + H2O | - |
Sulfolobus acidocaldarius | alpha-D-glucose | - |
? | |
| maltotriose + H2O | - |
Sulfolobus acidocaldarius | maltose + alpha-D-glucose | - |
? | |
| additional information | no activity towards cellobiose, starch and glycogen | Sulfolobus acidocaldarius | ? | - |
? | |
| additional information | no activity towards cellobiose, starch and glycogen | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
| nigerose + H2O | i.e. alpha-D-Glc-(1->3)-D-Glc | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
| panose + H2O | alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc | Sulfolobus acidocaldarius | maltose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | 12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
| dodecamer | 12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MalA | - |
Sulfolobus acidocaldarius |
| Saci1160 | locus name | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | 100 | - |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 110 | 80°C: about 60% of maximal activity, 110°C: about 80% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
half-life: 33.8 h | Sulfolobus acidocaldarius |
| 100 | - |
half-life: 10.6 h | Sulfolobus acidocaldarius |
| 105 | - |
half-life: 1.8 h | Sulfolobus acidocaldarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.7 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 372 | - |
maltohexaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 527 | - |
maltotetraose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 550 | - |
maltotriose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 611 | - |
maltopentaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 1278 | - |
maltose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | pH 4.0: about 35% of maximal activity, pH 7.0: about 40% of maximal activity | Sulfolobus acidocaldarius |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sulfolobus acidocaldarius | calculated from sequence | - |
5.9 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Sulfolobus acidocaldarius | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in maltose and starch metabolism | Sulfolobus acidocaldarius |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.2 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 716 | - |
maltohexaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 1597 | - |
maltose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 1759 | - |
maltotetraose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 1833 | - |
maltotriose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
| 1910 | - |
maltopentaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
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