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Literature summary for 3.2.1.20 extracted from
- Recombinant expression and characterization of Thermoanaerobacter tengcongensis thermostable alpha-glucosidase with regioselectivity for high yield isomaltooligosaccharides synthesis (2009), J. Microbiol. Biotechnol., 19, 1547-1556.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Tris | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.08 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 4.58 | - |
maltotriose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 6.31 | - |
maltose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 6.72 | - |
maltopentaose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 16.55 | - |
maltotetraose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 89000 | - |
3 * 89000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldanaerobacter subterraneus subsp. tengcongensis MB4 | Q8RDL1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 44.7fold after heating treatment, His-tag column affinity chromatography and gel-filtration | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.326 | - |
after purification | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
| 0.36 | - |
p-nitrophenyl-alpha-D-glucopyranoside | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
| 0.73 | - |
substrate dextrin | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
| 1.09 | - |
substrate maltotetraose | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
| 2.38 | - |
substrate maltopentaose | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
| 2.85 | - |
substrate maltotriose | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
| 3.26 | - |
substrate maltose | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | assay at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | 4-nitrophenol + D-glucose | - |
? | |
| dextrin + H2O | assay at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
| maltopentaose + H2O | assay at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
| maltose + H2O | assay at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
| maltotetraose + H2O | assay at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
| maltotriose + H2O | assay at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 89000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucosidase | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
| TtGluA | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
halflife at 60°C, 2 h | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.92 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 217.2 | - |
maltotriose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 253.5 | - |
maltose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 316 | - |
maltopentaose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 330.1 | - |
maltotetraose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.45 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 19.95 | - |
maltotetraose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 40.17 | - |
maltose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 47.01 | - |
maltopentaose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
| 47.43 | - |
maltotriose | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
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