Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.20 extracted from

  • da Silva, T.M.; Michelin, M.; Damasio, A.R.; Maller, A.; Almeida, F.B.; Ruller, R.; Ward, R.J.; Rosa, J.C.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.d.e..L.
    Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus (2009), Antonie van Leeuwenhoek, 96, 569-578.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 10 mM Aspergillus niveus
beta-mercaptoethanol 10 mM Aspergillus niveus
Fe2+ 10 mM Aspergillus niveus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
glycogen 0.04 mg/ml Aspergillus niveus
additional information
-
starch 0.07 mg/ml Aspergillus niveus
additional information
-
amylopectin 0.11 mg/ml Aspergillus niveus
additional information
-
maltose 0.57 mg/ml Aspergillus niveus
0.55
-
p-nitrophenyl alpha-D-glucopyranoside
-
Aspergillus niveus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus niveus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ag2+ inhibitor, 10 mM Aspergillus niveus
Fe2+ inhibitor, 10 mM Aspergillus niveus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
Bio-Sil-Sec-400 gel filtration column Aspergillus niveus
56000
-
SDS-PAGE Aspergillus niveus

Organism

Organism UniProt Comment Textmining
Aspergillus niveus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Fractogel ion-exchange chromatography and Sephacryl S-200 gel filtration Aspergillus niveus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
0.2 micromol/min/ml, substrate trehalose Aspergillus niveus
additional information
-
0.3 micromol/min/ml, substrate isomaltose Aspergillus niveus
additional information
-
0.4 micromol/min/ml, substrate beta-cyclodextrin Aspergillus niveus
additional information
-
1.2 micromol/min/ml, substrate p-nitrophenyl alpha-D-glucopyranoside Aspergillus niveus
additional information
-
2.7 micromol/min/ml, substrate maltose Aspergillus niveus
additional information
-
4.2 micromol/min/ml, substrate amylopectin Aspergillus niveus
additional information
-
4.2 micromol/min/ml, substrate maltooligosaccharide Aspergillus niveus
additional information
-
4.2 micromol/min/ml, substrate maltotriose Aspergillus niveus
additional information
-
4.3 micromol/min/ml, substrate maltotetraose Aspergillus niveus
additional information
-
4.3 micromol/min/ml, substrate starch Aspergillus niveus
additional information
-
4.4 micromol/min/ml, substrate amylose Aspergillus niveus
additional information
-
4.5 micromol/min/ml, substrate glycogen Aspergillus niveus
additional information
-
4.5 micromol/min/ml, substrate maltopentaose Aspergillus niveus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus 4-nitrophenol + D-glucose
-
?
amylopectin + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
?
glycogen + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
?
maltose + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
?
starch + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
?

Synonyms

Synonyms Comment Organism
alpha-glucosidase
-
Aspergillus niveus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Aspergillus niveus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 65 half-life at 60°C 104 min, half-life at 65°C 78 min Aspergillus niveus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
p-nitrophenyl alpha-D-glucopyranoside
-
Aspergillus niveus
13.66
-
maltose
-
Aspergillus niveus
24.5
-
glycogen
-
Aspergillus niveus
24.62
-
starch
-
Aspergillus niveus
25.48
-
amylopectin
-
Aspergillus niveus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Aspergillus niveus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8.5 acid-alkaline tolerant, remains stable after 2 h incubation Aspergillus niveus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
p-nitrophenyl alpha-D-glucopyranoside 0.96 1/s*mg*ml Aspergillus niveus
additional information
-
maltose 23.96 1/s*mg*ml Aspergillus niveus
additional information
-
amylopectin 231.64 1/s*mg*ml Aspergillus niveus
additional information
-
starch 351.71 1/s*mg*ml Aspergillus niveus
additional information
-
glycogen 612.5 1/s*mg*ml Aspergillus niveus