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Literature summary for 3.2.1.20 extracted from

  • Hall, B.G.; Pikis, A.; Thompson, J.
    Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations (2009), Mol. Biol. Evol., 26, 2487-2497.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.20

Activating Compound

Activating Compound Comment Organism Structure
NAD+
-
 Erwinia rhapontici

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Erwinia rhapontici
gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli Erwinia rhapontici

Protein Variants

Protein Variants Comment Organism
E173S site-directed mutagenesis Erwinia rhapontici
E173S mutation with loss of al alpha-glucosidase activity, but the mutant protein exhibits no alpha-galactosidase activtiy Erwinia rhapontici
E173S/I174V site-directed mutagenesis Erwinia rhapontici
E173S/I174V mutation with loss of al alpha-glucosidase activity, but the mutant protein exhibits no alpha-galactosidase activtiy Erwinia rhapontici
I174V site-directed mutagenesis Erwinia rhapontici
I174V mutation with loss of al alpha-glucosidase activity, but the mutant protein exhibits no alpha-galactosidase activtiy Erwinia rhapontici

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.75
-
 4-O-alpha-D-glucopyranosyl-D-fructose
-
 Erwinia rhapontici
2.52
-
 6-O-alpha-D-glucopyranosyl-D-fructofuranoside
-
 Erwinia rhapontici
5.31
-
 5-O-alpha-D-glucopyranosyl-D-fructose
-
 Erwinia rhapontici
7.31
-
 4-O-alpha-D-glucopyranosyl-D-sorbitol
-
 Erwinia rhapontici
8.58
-
 4-O-alpha-D-glucopyranosyl-D-glucopyranose
-
 Erwinia rhapontici
10.04
-
 3-O-alpha-D-glucopyranosyl-D-fructose
-
 Erwinia rhapontici
22.11
-
 1-O-alpha-D-glucopyranosyl-beta-D-fructofuranoside
-
 Erwinia rhapontici

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Erwinia rhapontici
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Erwinia rhapontici

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 SDS-PAGE Erwinia rhapontici

Organism

Organism UniProt Comment Textmining
Erwinia rhapontici
-
-
-
Erwinia rhapontici Q9AI65 strain DSM448, gene palH
-
Erwinia rhapontici DSM 448 Q9AI65 strain DSM448, gene palH
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chormatography and gel filtration chromatography Erwinia rhapontici
recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration Erwinia rhapontici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-alpha-D-glucopyranosyl-beta-D-fructofuranoside + H2O
-
 Erwinia rhapontici alpha-D-glucose + D-fructose
-
 ?
3-O-alpha-D-glucopyranosyl-D-fructose + H2O
-
 Erwinia rhapontici alpha-D-glucose + D-fructose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O assay at pH 7.5, 37° Erwinia rhapontici 4-nitrophenol + alpha-D-glucose
-
 ?
4-O-alpha-D-glucopyranosyl-D-fructose + H2O
-
 Erwinia rhapontici alpha-D-glucose + D-fructose
-
 ?
4-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O
-
 Erwinia rhapontici alpha-D-glucose + alpha-D-glucose
-
 ?
4-O-alpha-D-glucopyranosyl-D-sorbitol + H2O
-
 Erwinia rhapontici alpha-D-glucose + D-sorbitol
-
 ?
5-O-alpha-D-glucopyranosyl-D-fructose + H2O
-
 Erwinia rhapontici alpha-D-glucose + D-fructose
-
 ?
6-O-alpha-D-glucopyranosyl-D-fructofuranoside + H2O
-
 Erwinia rhapontici alpha-D-glucose + D-fructose
-
 ?
additional information an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism Erwinia rhapontici ?
-
 ?
additional information an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism Erwinia rhapontici DSM 448 ?
-
 ?

Synonyms

Synonyms Comment Organism
6-phospho-alpha-glucosidase
-
 Erwinia rhapontici
alpha-glucosid-specific GH4 enzyme
-
 Erwinia rhapontici
alpha-glucosidase
-
 Erwinia rhapontici
CHEI
-
 Erwinia rhapontici
More the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site Erwinia rhapontici
PalH
-
 Erwinia rhapontici
phospho-alpha-glucosidase
-
 Erwinia rhapontici

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Erwinia rhapontici
37
-
 assay at Erwinia rhapontici

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 4-O-alpha-D-glucopyranosyl-D-sorbitol
-
 Erwinia rhapontici
0.27
-
 1-O-alpha-D-glucopyranosyl-beta-D-fructofuranoside
-
 Erwinia rhapontici
0.37
-
 5-O-alpha-D-glucopyranosyl-D-fructose
-
 Erwinia rhapontici
0.38
-
 3-O-alpha-D-glucopyranosyl-D-fructose
-
 Erwinia rhapontici
0.45
-
 4-O-alpha-D-glucopyranosyl-D-fructose
-
 Erwinia rhapontici
0.58
-
 6-O-alpha-D-glucopyranosyl-D-fructofuranoside
-
 Erwinia rhapontici
0.85
-
 4-O-alpha-D-glucopyranosyl-D-glucopyranose
-
 Erwinia rhapontici

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Erwinia rhapontici

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Erwinia rhapontici