Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis ISW1214 cells | Geobacillus sp. HTA-462 |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the enzyme is determined to a 2.0 A resolution, by X-ray crystallography | Geobacillus sp. HTA-462 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucono-1,5-lactone | - |
Geobacillus sp. HTA-462 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Geobacillus sp. HTA-462 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. HTA-462 | Q33E90 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Geobacillus sp. HTA-462 |
Purification (Comment) | Organism |
---|---|
GSJ is purified to homogeneity, gel filtration | Geobacillus sp. HTA-462 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligomaltose + H2O | broad ability in transglycosylation activity on nonsugar acceptors | Geobacillus sp. HTA-462 | D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | GSJ forms a homodimer under low-ionic strength conditions, while all the structure-determined homologues are supposed to be monomeric proteins | Geobacillus sp. HTA-462 |
More | comparisons of the GSJ structure with that of other GH13 enzymes with different catalytic activities reveals that the catalytic cleft of GSJ is widely opened when compared with the homologues | Geobacillus sp. HTA-462 |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase | - |
Geobacillus sp. HTA-462 |
GH13 alpha-glucosidase | - |
Geobacillus sp. HTA-462 |
GSJ | - |
Geobacillus sp. HTA-462 |