Literature summary for 3.2.1.20 extracted from

Rolfsmeier, M.; Haseltine, C.; Bini, E.; Clark, A.; Blum, P.
Molecular characterization of the alpha-glucosidase gene (malA) from the hyperthermophilic archaeon Sulfolobus solfataricus (1998), J. Bacteriol., 180, 1287-1295.

Cloned(Commentary)

Cloned (Comment)	Organism
gene malA, library preparation, DNA and amino acid sequence determination and analysis, genetic structure and organization, sequences 3' to position -33, including a consensus archaeal TATA box, play an essential role in malA expression, detection of malA homologues, overview, phylogenetic analysis, expression of MalA in Escherichia coli revealing differences from the native enzyme in thermostability and electrophoretic behavior, overview	Saccharolobus solfataricus

Cloned (Comment)

Organism

gene malA, library preparation, DNA and amino acid sequence determination and analysis, genetic structure and organization, sequences 3' to position -33, including a consensus archaeal TATA box, play an essential role in malA expression, detection of malA homologues, overview, phylogenetic analysis, expression of MalA in Escherichia coli revealing differences from the native enzyme in thermostability and electrophoretic behavior, overview

Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
64.9	-	glycogen	pH 5.5, 85°C, recombinant enzyme	Saccharolobus solfataricus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Saccharolobus solfataricus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80500	-	x * 80500, about, sequence calculation	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
maltose + H2O	Saccharolobus solfataricus	-	alpha-D-glucose + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	P0CD66	malA; strains 98/2 and P2, gene malA	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycogen + H2O	recombinant enzyme	Saccharolobus solfataricus	alpha-D-glucose	-	?
maltose + H2O	-	Saccharolobus solfataricus	alpha-D-glucose + D-glucose	-	?

Subunits

Subunits	Comment	Organism
?	x * 80500, about, sequence calculation	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
MalA	-	Saccharolobus solfataricus
maltase	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	recombinant enzyme, substrate glycogen	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	recombinant enzyme, substrate glycogen	Saccharolobus solfataricus