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Literature summary for 3.2.1.20 extracted from

  • Chanchao, C.; Padoongsupalai, R.; Sangvanich, P.
    Expression and characterization of alpha-glucosidase III in the dwarf honeybee, Apis florea (Hymenoptera: Apoidea: Apidae) (2007), Insect Sci., 14, 283-293.
No PubMed abstract available
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis, semi-quantitative expression analysis, sequence comparison Apis florea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 gel filtration Apis florea
75000
-
 1 * 75000, SDS-PAGE Apis florea

Organism

Organism UniProt Comment Textmining
Apis florea A5JVZ2 dwarf honeybee
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 13.4fold by ammonium sulfate fractination, anion exchange chromatography, and gel filtration Apis florea

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression levels at different developmental stages, highest expressionlevel in forager bees, no detectable expression in eggs and only a low expression level in nurse bees, overview Apis florea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is active with sucrose Apis florea ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Apis florea

Synonyms

Synonyms Comment Organism
alpha-glucosidase III
-
 Apis florea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Apis florea
45
-
 in-gel activity assay Apis florea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Apis florea