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Literature summary for 3.2.1.20 extracted from

  • Ter Kuile, B.H.; Hrdy, I.; Sanchez, L.B.; Muller, M.
    Purification and specificity of two alpha-glucosidase isoforms of the parasitic protist Trichomonas vaginalis (2000), J. Eukaryot. Microbiol., 47, 440-442.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by phosphate Trichomonas vaginalis
Naphthyl acetate
-
Trichomonas vaginalis
p-chloromercuribenzoic acid
-
Trichomonas vaginalis
wheat amylase inhibitor
-
Trichomonas vaginalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
maltose isozyme 1, pH 4.5, 37°C Trichomonas vaginalis
2
-
maltose isozyme 2, pH 5.5, 37°C Trichomonas vaginalis

Organism

Organism UniProt Comment Textmining
Trichomonas vaginalis
-
parasitic protist, 2 isozymes 1 and 2
-

Purification (Commentary)

Purification (Comment) Organism
2 isozymes 1 and 2, isozyme 2 is purified 472fold Trichomonas vaginalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.9
-
purified isozyme 1, substrate 4-nitrophenyl alpha-D-glucopyranoside Trichomonas vaginalis
17
-
purified isozyme 2, substrate 4-nitrophenyl alpha-D-glucopyranoside Trichomonas vaginalis
30
-
purified isozyme 1, substrate maltose Trichomonas vaginalis
101
-
purified isozyme 2, substrate maltose Trichomonas vaginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Trichomonas vaginalis 4-nitrophenol + alpha-D-glucopyranose
-
?
isomaltose + H2O
-
Trichomonas vaginalis 2 alpha-D-glucose
-
?
maltoheptaose + H2O
-
Trichomonas vaginalis maltohexaose + D-glucose
-
?
maltohexaose + H2O
-
Trichomonas vaginalis maltopentaose + D-glucose
-
?
maltopentaose + H2O
-
Trichomonas vaginalis alpha-D-glucose + ?
-
?
maltose + H2O preferred substrate of both isozymes Trichomonas vaginalis alpha-D-glucose + D-glucose
-
?
maltotetraose + H2O
-
Trichomonas vaginalis alpha-D-glucose
-
?
maltotriose + H2O
-
Trichomonas vaginalis maltose + alpha-D-glucose
-
?
additional information substrate specificity, no activity of either isozyme with amylose and glycogen, no activity of isozyme 1 with amylopectin, starch, trehalose, panose, and lactose are a poor substrates for both isozymes, amylopectin is a poor substrate for isozyme 2 Trichomonas vaginalis ?
-
?
nigerose + H2O high activity Trichomonas vaginalis 2 alpha-D-glucose
-
?
turanose + H2O
-
Trichomonas vaginalis alpha-D-glucose + D-fructose
-
?

Subunits

Subunits Comment Organism
? x * 100000-105000, isozymes 1 and 2, SDS-PAGE Trichomonas vaginalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
isozyme 1 Trichomonas vaginalis
5.5
-
isozyme 2 Trichomonas vaginalis