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Literature summary for 3.2.1.20 extracted from

  • Nashiru, O.; Koh, S.; Lee, S.Y.; Lee, D.S.
    Novel alpha-glucosidase from extreme thermophile Thermus caldophilus GK24 (2001), J. Biochem. Mol. Biol., 34, 347-354.
No PubMed abstract available
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli Thermus caldophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Thermus caldophilus

Organism

Organism UniProt Comment Textmining
Thermus caldophilus
-
 enzyme TcaAG
-
Thermus caldophilus GK24
-
 enzyme TcaAG
-

Purification (Commentary)

Purification (Comment) Organism
78fold to homogeneity Thermus caldophilus

Reaction

Reaction Comment Organism Reaction ID
maltotetraose + 3 H2O = 4 alpha-D-glucose residues Asp198, Glu265, and Asp327 are involved in catalysis, His100 and His326 are involved in substrate binding Thermus caldophilus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
999.5
-
 purified enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside Thermus caldophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-glucopyranoside + H2O
-
 Thermus caldophilus 4-methylumbelliferol + alpha-D-glucopyranose
-
 ?
4-methylumbelliferyl alpha-D-glucopyranoside + H2O
-
 Thermus caldophilus GK24 4-methylumbelliferol + alpha-D-glucopyranose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Thermus caldophilus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Thermus caldophilus GK24 4-nitrophenol + alpha-D-glucopyranose
-
 ?
isomaltosaccharides + H2O hydrolysis of alpha-1,6-glycosidic bond of the nonreducing terminal unit Thermus caldophilus alpha-D-glucose
-
 ?
additional information no activity with cellobiose, laminaribiose, trehalose, strach, pullulan, lactose, maltose, leucrose, and gentibiose Thermus caldophilus ?
-
 ?
additional information no activity with cellobiose, laminaribiose, trehalose, strach, pullulan, lactose, maltose, leucrose, and gentibiose Thermus caldophilus GK24 ?
-
 ?
nigerose + H2O hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit Thermus caldophilus 2 alpha-D-glucose
-
 ?
nigerose + H2O hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit Thermus caldophilus GK24 2 alpha-D-glucose
-
 ?
panose + H2O hydrolysis of alpha-1,6-glycosidic bond of the nonreducing terminal unit Thermus caldophilus alpha-D-glucopyranose + alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,6-alpha-D-glucopyranosyl-1,4-D-glucopyranose
-
 ?
sucrose + H2O hydrolysis of alpha-1,2-glycosidic bond of the nonreducing terminal unit Thermus caldophilus alpha-D-glucose + D-fructose
-
 ?
turanose + H2O hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit Thermus caldophilus alpha-D-glucose + D-fructose
-
 ?
turanose + H2O hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit Thermus caldophilus GK24 alpha-D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Thermus caldophilus

Synonyms

Synonyms Comment Organism
TcaAG
-
 Thermus caldophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermus caldophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 stable up to, half-life 85 min Thermus caldophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Thermus caldophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 stable Thermus caldophilus