KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Mortierella alliacea | |
2.4 | - |
maltotetraose | pH 5.0, 37°C | Mortierella alliacea | |
2.7 | - |
maltotriose | pH 5.0, 37°C | Mortierella alliacea | |
2.8 | - |
maltopentaose | pH 5.0, 37°C | Mortierella alliacea | |
2.9 | - |
maltohexaose | pH 5.0, 37°C | Mortierella alliacea | |
3.5 | - |
maltose | pH 5.0, 37°C | Mortierella alliacea | |
4.9 | - |
maltoheptaose | pH 5.0, 37°C | Mortierella alliacea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Mortierella alliacea | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
1 * 61000 + 1 * 31000, SDS-PAGE | Mortierella alliacea |
61000 | - |
1 * 61000 + 1 * 31000, SDS-PAGE | Mortierella alliacea |
92000 | - |
gel filtration | Mortierella alliacea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mortierella alliacea | the organism does not possess alpha-amylase activity | ? | - |
? | |
additional information | Mortierella alliacea YN-15 | the organism does not possess alpha-amylase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alliacea | - |
- |
- |
Mortierella alliacea YN-15 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from culture supernatant, 33fold by ethanol precipitation and 4 chromatographic steps | Mortierella alliacea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
purified enzyme, substrate isomaltose | Mortierella alliacea |
3.4 | - |
purified enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside | Mortierella alliacea |
5.3 | - |
purified enzyme, substrate maltitol | Mortierella alliacea |
7 | - |
purified enzyme, substrate kojibiose | Mortierella alliacea |
8.3 | - |
purified enzyme, substrate amylose | Mortierella alliacea |
26.1 | - |
purified enzyme, substrate nigerose | Mortierella alliacea |
29.8 | - |
purified enzyme, substrate maltose | Mortierella alliacea |
30.2 | - |
purified enzyme, substrate glycogen | Mortierella alliacea |
34.3 | - |
purified enzyme, substrate amylopectin | Mortierella alliacea |
42.9 | - |
purified enzyme, substrate soluble starch | Mortierella alliacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Mortierella alliacea | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
amylopectin + H2O | high activity | Mortierella alliacea | ? | - |
? | |
amylose + H2O | n = 18 | Mortierella alliacea | alpha-D-glucose | - |
? | |
D-glucose + ethanol | transglucosylation activity in presence of ethanol with several substrates, e.g. maltose, kojibiose, nigerose, glycogen, starch, overview, the enzyme favors the forward reaction | Mortierella alliacea | ethyl-alpha-D-glucoside | - |
r | |
glycogen + H2O | high activity | Mortierella alliacea | alpha-D-glucose | - |
? | |
isomaltose + H2O | low activity | Mortierella alliacea | 2 D-glucose | - |
? | |
kojibiose + H2O | - |
Mortierella alliacea | alpha-D-glucose + D-glucose | - |
? | |
kojibiose + H2O | - |
Mortierella alliacea YN-15 | alpha-D-glucose + D-glucose | - |
? | |
maltitol + H2O | - |
Mortierella alliacea | ? | - |
? | |
maltoheptaose + H2O | - |
Mortierella alliacea | maltohexaose + D-glucose | - |
? | |
maltohexaose + H2O | - |
Mortierella alliacea | maltopentaose + D-glucose | - |
? | |
maltopentaose + H2O | - |
Mortierella alliacea | D-glucose + ? | - |
? | |
maltose + H2O | maltose is a glucosyl donor for transglycosylation activity, high activity | Mortierella alliacea | 2 D-glucose | - |
? | |
maltose + H2O | maltose is a glucosyl donor for transglycosylation activity, high activity | Mortierella alliacea YN-15 | 2 D-glucose | - |
? | |
maltotetraose + H2O | - |
Mortierella alliacea | maltotriose + D-glucose | - |
? | |
maltotriose + H2O | - |
Mortierella alliacea | maltose + D-glucose | - |
? | |
additional information | the organism does not possess alpha-amylase activity | Mortierella alliacea | ? | - |
? | |
additional information | dextran, pullulan, ethyl-alpha-D-glucoside, sucrose, and trehalose are poor substrates | Mortierella alliacea | ? | - |
? | |
additional information | the organism does not possess alpha-amylase activity | Mortierella alliacea YN-15 | ? | - |
? | |
additional information | dextran, pullulan, ethyl-alpha-D-glucoside, sucrose, and trehalose are poor substrates | Mortierella alliacea YN-15 | ? | - |
? | |
nigerose + H2O | high activity | Mortierella alliacea | 2 alpha-D-glucose | - |
? | |
nigerose + H2O | high activity | Mortierella alliacea YN-15 | 2 alpha-D-glucose | - |
? | |
starch + H2O | soluble starch, high activity, best substrate | Mortierella alliacea | alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 61000 + 1 * 31000, SDS-PAGE | Mortierella alliacea |
More | the subunits are stably aggregated to each other even at high salt concentrations of 0.5 M | Mortierella alliacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | formation of ethyl-alpha-D-glucose | Mortierella alliacea |
55 | - |
substrate maltose | Mortierella alliacea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mortierella alliacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
formation of ethyl-alpha-D-glucose | Mortierella alliacea |
5 | 6 | substrate maltose | Mortierella alliacea |