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Literature summary for 3.2.1.20 extracted from
- The primary structure of the subunit in Bacillus thermoamyloliquefaciens KP1071 molecular weight 540,000 homohexameric alpha-glucosidase II belonging to the glycosyl hydrolase family 31 (2000), Biosci. Biotechnol. Biochem., 64, 1379-1393.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of a genomic library, gene mapping, DNA and amino acid sequence determination and analysis, expression in Escherichia coli HB101 and DH5alpha | Bacillus thermoamyloliquefaciens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE | Bacillus thermoamyloliquefaciens |
| 91070 | - |
6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE | Bacillus thermoamyloliquefaciens |
| 530000 | - |
alpha-glucosidase II, native PAGE | Bacillus thermoamyloliquefaciens |
| 540000 | - |
alpha-glucosidase II, gel filtration | Bacillus thermoamyloliquefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thermoamyloliquefaciens | Q9F234 | 3 alpha-glucosidases I, II, and III | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant alpha-glucosidase II from Escherichia coli, 86fold | Bacillus thermoamyloliquefaciens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| maltotetraose + 3 H2O = 4 alpha-D-glucose | the enzyme has both exo-alpha-1,4-glucosidase and oligo-alpha-1,6-glucosidase activities | Bacillus thermoamyloliquefaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 64.7 | - |
purified alpha-glucosidase II | Bacillus thermoamyloliquefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | hydrolysis of alpha-1,6-bond | Bacillus thermoamyloliquefaciens | 2 D-glucose | - |
? |  |
| maltose + H2O | hydrolysis of alpha-1,4-bond | Bacillus thermoamyloliquefaciens | 2 D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE | Bacillus thermoamyloliquefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme belongs to the family 31 of glycosyl hydrolases | Bacillus thermoamyloliquefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
alpha-glucosidase II | Bacillus thermoamyloliquefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
alpha-glucosidase II, stable up to for 10 min | Bacillus thermoamyloliquefaciens |
| 80 | - |
alpha-glucosidase II, half-life 10 min | Bacillus thermoamyloliquefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Bacillus thermoamyloliquefaciens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus thermoamyloliquefaciens | alpha-glucosidase II, isoelectric focusing | - |
4.9 |
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