Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli, phylogenetic analysis | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
maltotriose | pH 5.5, 45°C | Aspergillus nidulans | |
0.51 | - |
maltose | pH 5.5, 45°C | Aspergillus nidulans | |
0.64 | - |
maltotetraose | pH 5.5, 45°C | Aspergillus nidulans | |
0.81 | - |
nigerose | pH 5.5, 45°C | Aspergillus nidulans | |
0.89 | - |
trehalose | pH 5.5, 45°C | Aspergillus nidulans | |
0.96 | - |
maltopentaose | pH 5.5, 45°C | Aspergillus nidulans | |
1.3 | - |
isomaltose | pH 5.5, 45°C | Aspergillus nidulans | |
1.3 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 45°C | Aspergillus nidulans | |
1.9 | - |
kojibiose | pH 5.5, 45°C | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 74000 + 1 * 55000, SDS-PAGE | Aspergillus nidulans |
74000 | - |
1 * 74000 + 1 * 55000, SDS-PAGE | Aspergillus nidulans |
130000 | - |
gel filtration | Aspergillus nidulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q9C1S7 | alpha-glucosidase B, i.e. AgdB | - |
Aspergillus nidulans ABPU1 | Q9C1S7 | alpha-glucosidase B, i.e. AgdB | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus nidulans |
Purification (Comment) | Organism |
---|---|
52fold to homogeneity by ammonium sulfate precipitation, dialysis, ion exchange and gel chromatography | Aspergillus nidulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.6 | - |
purified enzyme | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Aspergillus nidulans | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
isomaltose + H2O | - |
Aspergillus nidulans | 2 D-glucose | - |
? | |
isomaltose + H2O | - |
Aspergillus nidulans ABPU1 | 2 D-glucose | - |
? | |
kojibiose + H2O | - |
Aspergillus nidulans | alpha-D-glucose + D-glucose | - |
? | |
kojibiose + H2O | - |
Aspergillus nidulans ABPU1 | alpha-D-glucose + D-glucose | - |
? | |
maltopentaose + H2O | - |
Aspergillus nidulans | D-glucose + ? | - |
? | |
maltose + H2O | - |
Aspergillus nidulans | 2 D-glucose | - |
? | |
maltose + H2O | - |
Aspergillus nidulans ABPU1 | 2 D-glucose | - |
? | |
maltotetraose + H2O | - |
Aspergillus nidulans | maltotriose + D-glucose | - |
? | |
maltotriose + H2O | best substrate | Aspergillus nidulans | maltose + D-glucose | - |
? | |
additional information | the enzyme shows strong transglycosylation activity | Aspergillus nidulans | ? | - |
? | |
additional information | the enzyme shows strong transglycosylation activity | Aspergillus nidulans ABPU1 | ? | - |
? | |
nigerose + H2O | - |
Aspergillus nidulans | 2 alpha-D-glucose | - |
? | |
nigerose + H2O | - |
Aspergillus nidulans ABPU1 | 2 alpha-D-glucose | - |
? | |
trehalose + H2O | - |
Aspergillus nidulans | alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 74000 + 1 * 55000, SDS-PAGE | Aspergillus nidulans |
Synonyms | Comment | Organism |
---|---|---|
AgdB | - |
Aspergillus nidulans |
alpha-glucosidase B | - |
Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Aspergillus nidulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
over 90% retained activity up to | Aspergillus nidulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Aspergillus nidulans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | loss of less than 10% activity | Aspergillus nidulans |