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Literature summary for 3.2.1.20 extracted from

  • Nakao, M.; Nakayama, T.; Harada, M.; Kakudo, A.; Ikemoto, H.; Kobayashi, S.; Shibano, Y.
    Purification and characterization of a Bacillus sp. SAM1606 thermostable alpha-glucosidase with transglucosylation activity (1994), Appl. Microbiol. Biotechnol., 41, 337-343.
    View publication on PubMed
Search for more literature for 3.2.1.20

Application

Application Comment Organism
nutrition high thermostability of the enzyme permits the transglucosylation reaction at high temperatures, which is beneficial for continous production of oligosaccharides from sucrose Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM Bacillus sp. (in: Bacteria)
Cu2+ 0.1 mM Bacillus sp. (in: Bacteria)
Pb2+ 0.1 mM Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 isomaltose
-
 Bacillus sp. (in: Bacteria)
3.2
-
 trehalose
-
 Bacillus sp. (in: Bacteria)
4.1
-
 isomaltulose
-
 Bacillus sp. (in: Bacteria)
5.3
-
 sucrose
-
 Bacillus sp. (in: Bacteria)
6.7
-
 isomaltotriose
-
 Bacillus sp. (in: Bacteria)
8.7
-
 turanose
-
 Bacillus sp. (in: Bacteria)
10.5
-
 nigerose
-
 Bacillus sp. (in: Bacteria)
10.8
-
 maltose
-
 Bacillus sp. (in: Bacteria)
13.3
-
 maltotriose
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 SAM1606
-
Bacillus sp. (in: Bacteria) SAM1606
-
 SAM1606
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81.23
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O
-
 Bacillus sp. (in: Bacteria) 2 alpha-D-glucose
-
 ?
isomaltose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 2 alpha-D-glucose
-
 ?
isomaltotriose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose
-
 ?
isomaltotriose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 alpha-D-glucose
-
 ?
maltose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + D-glucose
-
 ?
maltose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 alpha-D-glucose + D-glucose
-
 ?
maltotriose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
additional information transglucosidase activity Bacillus sp. (in: Bacteria) ?
-
 ?
additional information transglucosidase activity Bacillus sp. (in: Bacteria) SAM1606 ?
-
 ?
nigerose + H2O
-
 Bacillus sp. (in: Bacteria) 2 alpha-D-glucose
-
 ?
nigerose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 2 alpha-D-glucose
-
 ?
palatinose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + D-fructose
-
 ?
sucrose
-
 Bacillus sp. (in: Bacteria) oligosaccharides containing theanderose ?
sucrose + H2O
-
 Bacillus sp. (in: Bacteria) D-fructose + alpha-D-glucose
-
 ?
trehalose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose
-
 ?
turanose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 60°C: about 35% of maximal activity, 80°C: about 70% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 pH 7.2, 10 min, stable up to Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
54.6
-
 sucrose
-
 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Bacillus sp. (in: Bacteria)